| The study of apoptotic bifunctional effects in relationship between host and parasite in cystic echinococcosis: a new approach to suppression and survival of hydatid cyst. | |
| | |
MedLine Citation:
|
PMID: 22167369 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Cystic echinococcosis (hydatidosis) is a zoonotic helminthic disease of human and other intermediated hosts wherein infection is caused by the larval stages of tapeworm Echinococcus granulosus. Growth of the larval stage is formed throughout the internal organs, the liver and lung, causing their destruction. Important pathways are unknown about suppression and survival of cysts in human body. In this study, apoptotic bifunctional effects are evaluated in relationship between host and parasite in cystic echinococcosis. Human lymphocytes were treated with hydatid fluid (HF). After 6 h of exposure, caspase-3 activity was measured by fluorometric assay in the HF-treated lymphocytes and control cells. Also, the expression of Bax (as pro-apoptotic protein) and Bcl-2 (an anti-apoptotic protein) mRNA was assessed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) after 12 h of exposure. For surveying of apoptosis-inducing ligands TNF-related apoptosis-inducing ligand and Fas-L, germinal layer and accompaniment peripheral tissues as healthy control were separated by scalpel from each cyst in sterile condition, then were assess by semiquantitative RT-PCR method in mRNA expression. Both the ratio of Bax/Bcl-2 mRNA expression and caspase-3 activity were higher in the fertile fluid-treated lymphocytes relative to infertile fluid-treated lymphocytes and control group versus the expression level of apoptosis-inducing ligands having a relatively high level in germinal layer of infertile cyst in comparison to fertile cyst and healthy tissue. Apoptosis of germinal layer of fertile cysts is possibly one of the suppression mechanisms in hydatidosis patients, in contrast to lymphocytes apoptosis by modulator of hydatid fluid, one of the hydatid cyst survival mechanisms. |
| | |
Authors:
|
Adel Spotin; Monireh Mokhtari Amir Majdi; Mojtaba Sankian; Abdolreza Varasteh |
Related Documents
:
|
18759749 - Secretion of interferon-gamma by human macrophages demonstrated at the single-cell leve... 17259169 - Presenilin/gamma-secretase-mediated cleavage regulates association of leukocyte-common ... 21612789 - Characterization of apoptosis pathways (intrinsic and extrinsic) in lymphoid tissues of... 11163659 - Immunostimulatory effect of il-18-encoding plasmid in dna vaccination against murine sc... 21782589 - Cytotoxicity and apoptosis induction of sodium fluoride in human promyelocytic leukemia... 12871129 - Interferon-gamma-induced conversion of tryptophan: immunologic and neuropsychiatric asp... 7662099 - Ceramide: an endogenous regulator of apoptosis and growth suppression. 16920499 - Migratory fate and differentiation of blood monocyte subsets. 8144939 - Regulatory mechanisms for the expression of type iv collagenases/gelatinases in murine ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-14 |
Journal Detail:
|
Title: Parasitology research Volume: - ISSN: 1432-1955 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8703571 Medline TA: Parasitol Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran, adelespotin@gmail.com. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular expression and characterization of a novel protein phosphatase 2A gene from Clonorchis sin...
Next Document: Larvicidal efficacy of Adhatoda vasica (L.) Nees against the bancroftian filariasis vector Culex qui...