Document Detail


A study of the acid phosphatase of Treponema denticola.
MedLine Citation:
PMID:  2085369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study describes some of the properties of the acid phosphatase of the potential periodontopathogen Treponema denticola. The highest enzyme activity was found in 87 h old cells. Two optimum pHs for enzyme activity were detected, one at pH 4.8 and another at pH 6.2. Divalent cations did not influence the acid phosphatase of T. denticola. The anion F- added in the form of NaF and at a level greater than 20 micrograms/ml F- diminished the activity of the acid phosphatase of intact cells of T. denticola. The addition of 10 micrograms/ml F- as SnF2 induced a statistically significant reduction of acid phosphatase activity. The apparent Km for the acid phosphatase was 7.3 mM with p-nitrophenyl phosphate as substrate. Fluoride appeared to be a noncompetitive inhibitor of the enzyme with an apparent Ki of 0.3 mM. Acid phosphatase may be released partially in osmotic shock fluids. Also, 7-diazonium-1, 3-naphthalene disulfonate, which is incapable of penetrating the bacterial permeability barrier and is known to inactivate enzymes found in the bacterial periplasmic place, suppressed the activity of the acid phosphatase in intact cells of T. denticola.
Authors:
C A Norton Hughes; W W Yotis
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Zentralblatt für Bakteriologie : international journal of medical microbiology     Volume:  274     ISSN:  0934-8840     ISO Abbreviation:  Zentralbl. Bakteriol.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-05-20     Completed Date:  1991-05-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9203851     Medline TA:  Zentralbl Bakteriol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  195-202     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Loyola University Stritch School of Medicine, Maywood, IL 60153.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / antagonists & inhibitors,  chemistry*,  metabolism
Fluorides / pharmacology*
Humans
Hydrogen-Ion Concentration
Kinetics
Treponema / enzymology*
Grant Support
ID/Acronym/Agency:
DE08125/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Fluorides; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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