Document Detail

A study of the Candida albicans cell wall proteome.
MedLine Citation:
PMID:  18712765     Owner:  NLM     Status:  MEDLINE    
Considering the importance of proteins in the structure and function of the cell wall of Candida albicans, we analyzed the cell wall subproteome of this important human pathogen by LC coupled to MS (LC-MS) using different protein extraction procedures. The analyzed samples included material extracted by hydrogen fluoride-pyridine (HF-pyridine), and whole SDS-extracted cell walls. The use of this latter innovative procedure gave similar data as compared to the analysis of HF-pyridine extracted proteins. A total of 21 cell wall proteins predicted to contain a signal peptide were identified, together with a high content of potentially glycosylated Ser/Thr residues, and the presence of a GPI motif in 19 of them. We also identified 66 "atypical" cell wall proteins that lack the above-mentioned characteristics. After tryptic removal of the most accessible proteins in the cell wall, several of the same expected GPI proteins and the most commonly found "atypical" wall proteins were identified. This result suggests that proteins are located not only at the cell wall surface, but are embedded within the cell wall itself. These results, which include new identified cell wall proteins, and comparison of proteins in blastospore and mycelial walls, will help to elucidate the C. albicans cell wall architecture.
Luis Castillo; Enrique Calvo; Ana I Martínez; José Ruiz-Herrera; Eulogio Valentín; Juan A Lopez; Rafael Sentandreu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  8     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-16     Completed Date:  2008-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3871-81     Citation Subset:  IM    
Departament de Microbiología i Ecología, Facultat de Farmacia, Universitat de València, Burjassot, Spain.
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MeSH Terms
Candida albicans / metabolism*
Cell Wall / metabolism*
Fungal Proteins / metabolism*
Hydrofluoric Acid
Proteome / metabolism*
Sodium Dodecyl Sulfate
Tandem Mass Spectrometry
Reg. No./Substance:
0/Fungal Proteins; 0/Proteome; 0/Pyridines; 0/Solvents; 110-86-1/pyridine; 151-21-3/Sodium Dodecyl Sulfate; 7664-39-3/Hydrofluoric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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