| The structure of the specific capsular polysaccharide of Rhodococcus equi serotype 4. | |
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MedLine Citation:
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PMID: 10573859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The specific capsular polysaccharide produced by Rhodococcus equi serotype 4 was found to be a high-molecular-weight acidic polymer composed of D-glucose, D-mannose, pyruvic acid and a previously unidentified 5-amino-3,5-dideoxynonulosonic (rhodaminic) acid in the proportions 2:1:1:1. Structural analysis, employing a combination of microanalytical methods, nuclear magnetic resonance spectroscopy, and mass spectrometric techniques, established that the polysaccharide consisted of linear repeating tetrasaccharide units having the sequence of residues shown below. In the native polysaccharide, the rhodaminic acid residues were present as their acetamido derivatives (RhoANAc) and carried 1-carboxyethylidene groups that bridged the O-7 and O-9 positions. Treatment of the capsular polysaccharide with dilute acetic acid and/or anhydrous hydrogen fluoride under hydrolytic/solvolytic conditions, resulted in the formation of four different oligosaccharide species. The 1H and 13C NMR resonances of these oligosaccharide fragments and of the native serotype 4 capsular polysaccharides were fully assigned by homo- and heteronuclear chemical shift correlation methods. |
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Authors:
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W B Severn; J C Richards |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Carbohydrate research Volume: 320 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 1999 Aug |
Date Detail:
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Created Date: 2000-01-27 Completed Date: 2000-01-27 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 209-22 Citation Subset: IM |
Affiliation:
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Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada. severnw@agresearch.cri.nz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Conformation Carbohydrate Sequence Magnetic Resonance Spectroscopy Molecular Sequence Data Polysaccharides / chemistry*, isolation & purification Polysaccharides, Bacterial / chemistry, isolation & purification Rhodococcus equi / chemistry* Serotyping Spectrometry, Mass, Fast Atom Bombardment |
| Chemical | |
Reg. No./Substance:
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0/Polysaccharides; 0/Polysaccharides, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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