Document Detail

The structure of the specific capsular polysaccharide of Rhodococcus equi serotype 4.
MedLine Citation:
PMID:  10573859     Owner:  NLM     Status:  MEDLINE    
The specific capsular polysaccharide produced by Rhodococcus equi serotype 4 was found to be a high-molecular-weight acidic polymer composed of D-glucose, D-mannose, pyruvic acid and a previously unidentified 5-amino-3,5-dideoxynonulosonic (rhodaminic) acid in the proportions 2:1:1:1. Structural analysis, employing a combination of microanalytical methods, nuclear magnetic resonance spectroscopy, and mass spectrometric techniques, established that the polysaccharide consisted of linear repeating tetrasaccharide units having the sequence of residues shown below. In the native polysaccharide, the rhodaminic acid residues were present as their acetamido derivatives (RhoANAc) and carried 1-carboxyethylidene groups that bridged the O-7 and O-9 positions. Treatment of the capsular polysaccharide with dilute acetic acid and/or anhydrous hydrogen fluoride under hydrolytic/solvolytic conditions, resulted in the formation of four different oligosaccharide species. The 1H and 13C NMR resonances of these oligosaccharide fragments and of the native serotype 4 capsular polysaccharides were fully assigned by homo- and heteronuclear chemical shift correlation methods.
W B Severn; J C Richards
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  320     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  2000-01-27     Completed Date:  2000-01-27     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  209-22     Citation Subset:  IM    
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada.
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MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Polysaccharides / chemistry*,  isolation & purification
Polysaccharides, Bacterial / chemistry,  isolation & purification
Rhodococcus equi / chemistry*
Spectrometry, Mass, Fast Atom Bombardment
Reg. No./Substance:
0/Polysaccharides; 0/Polysaccharides, Bacterial

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