Document Detail


The structure of a polysaccharide from infectious strains of Burkholderia cepacia.
MedLine Citation:
PMID:  11553353     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structure of an acidic exopolysaccharide (EPS) from eight strains of Burkholderia cepacia has been investigated by methylation and sugar analysis, periodate oxidation-Smith degradation, and partial acid-hydrolysis. An enzyme preparation obtained from the same organisms producing the EPS was also used to depolymerize the polysaccharide. Detailed NMR studies of the chemical and enzymatic degradation products showed that this EPS consists of a highly branched heptasaccharide-repeating unit with the following structure: [abstract: see text]. About three O-acetyl groups per repeating unit are present at undetermined positions.
Authors:
A Linker; L R Evans; G Impallomeni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  335     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-12     Completed Date:  2001-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  45-54     Citation Subset:  IM    
Affiliation:
Veterans Affairs (VA), Salt Lake City Health Care System, 500 Foothill Boulevard (151E), Salt Lake City, UT 84124, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopolymers / chemistry,  metabolism
Burkholderia cepacia / chemistry*,  classification*,  enzymology,  physiology
Carbohydrate Conformation
Carbohydrate Sequence
Cattle
Glucose Oxidase / metabolism
Glucuronidase / metabolism
Hydrolysis
Magnetic Resonance Spectroscopy
Methylation
Oxidation-Reduction
Periodic Acid / metabolism
Polysaccharides, Bacterial / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Biopolymers; 0/Polysaccharides, Bacterial; 10450-60-9/Periodic Acid; 7790-28-5/metaperiodate; EC 1.1.3.4/Glucose Oxidase; EC 3.2.1.31/Glucuronidase

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