| The structure and function of Saccharomyces cerevisiae proteinase A. | |
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MedLine Citation:
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PMID: 17447722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Saccharomyces cerevisiae proteinase A (saccharopepsin; EC 3.4.23.25) is a member of the aspartic proteinase superfamily (InterPro IPR001969), which are proteolytic enzymes distributed among a variety of organisms. Targeted to the vacuole as a zymogen, its activation at acidic pH can occur by two different pathways, a one-step process to release mature proteinase A, involving the intervention of proteinase B, or a step-wise pathway via the autoactivation product known as pseudo-proteinase A. Once active, S. cerevisiae proteinase A is essential to the activities of other yeast vacuolar hydrolases, including proteinase B and carboxypeptidase Y. The mature enzyme is bilobal, with each lobe providing one of the two catalytically essential aspartic acid residues in the active site. The crystal structure of free proteinase A reveals that the flap loop assumes an atypical position, pointing directly into the S(1) pocket of the enzyme. With regard to hydrolysis, proteinase A has a preference for hydrophobic residues with Phe, Leu or Glu at the P1 position and Phe, Ile, Leu or Ala at P1', and is inhibited by IA(3), a natural and highly specific inhibitor produced by S. cerevisiae. This review is the first comprehensive review of S. cerevisiae PrA. |
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Authors:
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Charity L Parr; Robert A B Keates; Brian C Bryksa; Masahiro Ogawa; Rickey Y Yada |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Yeast (Chichester, England) Volume: 24 ISSN: 0749-503X ISO Abbreviation: Yeast Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-04 Completed Date: 2007-09-07 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8607637 Medline TA: Yeast Country: England |
Other Details:
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Languages: eng Pagination: 467-80 Citation Subset: IM |
Affiliation:
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Department of Food Science, University of Guelph, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Aspartic Acid Endopeptidases / chemistry*, genetics, metabolism* Gene Expression Regulation, Fungal Models, Molecular Molecular Sequence Data Saccharomyces cerevisiae / chemistry, enzymology*, genetics Saccharomyces cerevisiae Proteins / chemistry, genetics, metabolism Structure-Activity Relationship Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Saccharomyces cerevisiae Proteins; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.23.19/aspergillopepsin II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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