Document Detail


The structure of the carbohydrate backbone of the core-lipid A region of the lipopolysaccharide from Proteus mirabilis serotype O28.
MedLine Citation:
PMID:  11117318     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The following structure of the lipid A-core region of the lipopolysaccharide (LPS) from Proteus mirabilis serotype O28 was determined using NMR, MS, and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of LPS, and of the products of alkaline deacylation of the LPS: carbohydrate sequence [see text] where S = beta-GalALys (amide of beta-D-galactopyranosyluronic acid with the alpha-amino group of L-lysine) or beta-GalALys4PEtN are present in the ratio of approximately 1:1. beta-GalA and Ara4N (indicated by bold italics) are present in non stoichometric amount. All sugars are present in the pyranose form and all except L-Ara4N have the D configuration.
Authors:
E Vinogradov; J Radziejewska-Lebrecht
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  329     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-14     Completed Date:  2001-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  351-7     Citation Subset:  IM    
Affiliation:
Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada. evguenii.vinogradov@nrc.ca
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial / chemistry*
Carbohydrate Conformation
Carbohydrate Sequence
Hydrolysis
Lipid A / chemistry
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Sequence Data
Oligosaccharides / chemistry*,  isolation & purification
Proteus / chemistry,  classification
Proteus mirabilis / chemistry*,  classification
Serotyping
Species Specificity
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Lipid A; 0/Lipopolysaccharides; 0/Oligosaccharides

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