| The structure of the basement membrane of human lymph node high endothelial venules: an ultrastructural, histochemical and immunocytochemical study. | |
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MedLine Citation:
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PMID: 3536803 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The structure of the basement membrane of the high endothelium of reactive human lymph nodes was investigated by techniques selective for carbohydrates (periodic acid-Schiff; critical electrolyte concentration staining with Alcian Blue; lectin histochemistry), specific proteins (immunohistochemistry for laminin and fibronectin) and by conventional techniques of light and transmission electron microscopy. Adjacent small lymphocytes were assigned to B and T cell subsets by use of monoclonal antibodies and they were analysed for non-specific esterase, beta-glucuronidase, beta-N-acetylglucaminidase and proteolytic activities. The basement membranes were shown to be distinctive and to contain three layers, of differing laminin, glycosaminoglycan and glycoprotein oligosaccharide content. Certain lymphocytes (probably T) contained enzymes potentially able to degrade some components of these basement membranes. |
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Authors:
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A J Freemont; R W Stoddart; F Steven; C J Jones; S Matthews |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Histochemical journal Volume: 18 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 1986 Aug |
Date Detail:
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Created Date: 1987-01-05 Completed Date: 1987-01-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 421-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Basement Membrane
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analysis,
ultrastructure Biological Transport Endothelium / ultrastructure Histocytochemistry Humans Immunoenzyme Techniques Lymph Nodes / ultrastructure Lymphatic System / ultrastructure* Lymphocytes / cytology*, metabolism |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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