Document Detail


The structure of the Ca2+ ATPase as revealed by electron microscopy and image processing of ordered arrays.
MedLine Citation:
PMID:  6232396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two-dimensional ordered arrays of the membrane-bound Ca2+ ATPase, were formed over a wide range of conditions (i.e., pH, ionic strength, temperature) in the presence of vanadate, and studied by electron microscopy and image processing. These ordered tubular and spherical membrane vesicles of Ca2+ ATPase could also be formed with approximately one bound ATP and between one and two nonchelatable Ca2+ bound. The tubular arrays ranged between 1 and 10 microns in length and had an average flattened diameter of 90 nm, as observed in negatively stained preparations. The basic building blocks of these ordered arrays appear to be linear ribbons of Ca2+ ATPase dimers. Fourier analysis of electron micrographs of these flattened tubes revealed a near-rectangular lattice (lattice angle 73.3 +/- 4.6 degrees with average lattice constants of a = 6.2 +/- 0.25 nm, and b = 11.5 +/- 0.30 nm). The double-stranded ribbons (i.e., parallel to a) are inclined by 56 +/- 3.7 degrees relative to the tube axis in a right-handed sense, as determined from freeze-dried metal-shadowed specimens. Computer averaging of negatively stained arrays reveals a crystallographic dimer of stain-excluding matter. The dimensions of each monomer within this dimer are consistent with established structural parameters, leading us to believe a form of the Ca2+ ATPase, capable of binding at least one ATP and of binding Ca2+ ions, may exist as a dimer in the sarcoplasmic reticulum.
Authors:
E L Buhle; B E Knox; E Serpersu; U Aebi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of ultrastructure research     Volume:  85     ISSN:  0022-5320     ISO Abbreviation:  J. Ultrastruct. Res.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-06-20     Completed Date:  1984-06-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376344     Medline TA:  J Ultrastruct Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  186-203     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium-Transporting ATPases*
Chemistry / methods
Microscopy, Electron
Muscles / cytology
Rabbits
Sarcoplasmic Reticulum / enzymology
Grant Support
ID/Acronym/Agency:
GM27765/GM/NIGMS NIH HHS; GM28795/GM/NIGMS NIH HHS; GM31940/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 3.6.1.8/Calcium-Transporting ATPases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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