Document Detail


A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification.
MedLine Citation:
PMID:  25109293     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used. We have compared the performance of conventional Taq and Bst DNA polymerases to a novel Taq DNA polymerase mutant (SD DNA polymerase), which has a strong strand displacement activity, in PCR (including amplification of GC-rich and complex secondary structure templates), long-range PCR (LR PCR), loop-mediated amplification (LAMP), and polymerase chain displacement reaction (PCDR). Our results demonstrate that the strand displacement activity of SD DNA polymerase, in combination with the robust polymerase activity, provides a notable improvement in the sensitivity and efficiency of all these methods.
Authors:
Konstantin B Ignatov; Ekaterina V Barsova; Arkady F Fradkov; Konstantin A Blagodatskikh; Tatiana V Kramarova; Vladimir M Kramarov
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Publication Detail:
Type:  Journal Article     Date:  2014-08-01
Journal Detail:
Title:  BioTechniques     Volume:  57     ISSN:  1940-9818     ISO Abbreviation:  BioTechniques     Publication Date:  2014  
Date Detail:
Created Date:  2014-08-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  England    
Other Details:
Languages:  eng     Pagination:  81-7     Citation Subset:  IM    
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