Document Detail


A straightforward route to the synthesis of a surface-enhanced Raman scattering probe for targeting transferrin receptor-overexpressed cells.
MedLine Citation:
PMID:  20683135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A tumor cell targeting surface-enhanced Raman scattering (SERS) probe has been successfully synthesized by using p-mercaptobenzoic acid (pMBA) as both the SERS reporter and the conjugation agent for attaching transferrin molecules, which shows experimentally the targeting ability for transferrin receptor-overexpressed HeLa cells and exhibits strong SERS signals when being incubated inside cells. To prove that the uptake of such a SERS probe is through a Tf-receptor-mediated endocytosis process, two control experiments: (1) HeLa cells being incubated with the probe at 4 degrees C and (2) HeLa cells being pre-blocked with free transferrin at 37 degrees C, were employed. The difference of SERS intensity between the transferrin-overexpressed HeLa cells and transferrin-pre-blocked HeLa cells indicates that the probe has the potential to selectively target tumor cells.
Authors:
Jing Yang; Zhuyuan Wang; Xuebin Tan; Jin Li; Chunyuan Song; Ruohu Zhang; Yiping Cui
Related Documents :
1472635 - The nuclear autoimmune antigen ku is also present on the cell surface.
17082585 - E2-2 regulates the expansion of pro-b cells and follicular versus marginal zone decisions.
18842625 - Improved cell-penetrating peptide-pna conjugates for splicing redirection in hela cells...
13385405 - Growth characteristics of tubercle bacilli and certain other mycobacteria in hela cells.
25118635 - A stem cell medium containing neural stimulating factor induces a pancreatic cancer ste...
11861865 - Selectable subgenomic and genome-length dicistronic rnas derived from an infectious mol...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-04
Journal Detail:
Title:  Nanotechnology     Volume:  21     ISSN:  1361-6528     ISO Abbreviation:  Nanotechnology     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-05     Completed Date:  2011-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  345101     Citation Subset:  IM    
Affiliation:
Advanced Photonics Center, Southeast University, Nanjing 210096, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Survival
Endocytosis
Hela Cells
Humans
Metal Nanoparticles / ultrastructure
Molecular Probes / chemical synthesis*
Receptors, Transferrin / genetics,  metabolism*
Silver / metabolism
Spectrum Analysis, Raman / methods*
Substrate Specificity
Surface Properties
Transferrin / metabolism
Chemical
Reg. No./Substance:
0/Molecular Probes; 0/Receptors, Transferrin; 11096-37-0/Transferrin; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Single-step generation of fluorophore-encapsulated gold nanoparticle core-shell materials.
Next Document:  Temperature dependence of energy dissipation on NaCl(001) in non-contact atomic force microscopy.