Document Detail

A straightforward route to the synthesis of a surface-enhanced Raman scattering probe for targeting transferrin receptor-overexpressed cells.
MedLine Citation:
PMID:  20683135     Owner:  NLM     Status:  MEDLINE    
A tumor cell targeting surface-enhanced Raman scattering (SERS) probe has been successfully synthesized by using p-mercaptobenzoic acid (pMBA) as both the SERS reporter and the conjugation agent for attaching transferrin molecules, which shows experimentally the targeting ability for transferrin receptor-overexpressed HeLa cells and exhibits strong SERS signals when being incubated inside cells. To prove that the uptake of such a SERS probe is through a Tf-receptor-mediated endocytosis process, two control experiments: (1) HeLa cells being incubated with the probe at 4 degrees C and (2) HeLa cells being pre-blocked with free transferrin at 37 degrees C, were employed. The difference of SERS intensity between the transferrin-overexpressed HeLa cells and transferrin-pre-blocked HeLa cells indicates that the probe has the potential to selectively target tumor cells.
Jing Yang; Zhuyuan Wang; Xuebin Tan; Jin Li; Chunyuan Song; Ruohu Zhang; Yiping Cui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-04
Journal Detail:
Title:  Nanotechnology     Volume:  21     ISSN:  1361-6528     ISO Abbreviation:  Nanotechnology     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-05     Completed Date:  2011-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  345101     Citation Subset:  IM    
Advanced Photonics Center, Southeast University, Nanjing 210096, People's Republic of China.
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MeSH Terms
Cell Survival
Hela Cells
Metal Nanoparticles / ultrastructure
Molecular Probes / chemical synthesis*
Receptors, Transferrin / genetics,  metabolism*
Silver / metabolism
Spectrum Analysis, Raman / methods*
Substrate Specificity
Surface Properties
Transferrin / metabolism
Reg. No./Substance:
0/Molecular Probes; 0/Receptors, Transferrin; 11096-37-0/Transferrin; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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