| A stochastic model of temporally regulated generation of oligodendrocytes in cell culture. | |
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MedLine Citation:
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PMID: 10361805 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The results of our previous analyses suggest that O-2A progenitor cells become competent for differentiation in vitro after they complete a certain number of critical mitotic cycles. The number of critical cycles varies from clone to clone and should be thought of as a random variable. We propose an approach to the analysis of oligodendrocyte generation in vitro based on a stochastic model allowing for an arbitrary distribution of this random variable with a finite support. When applied to experimental data on clonal growth and differentiation of purified O-2A progenitor cells obtained from optic nerves of 1 and 7 day-old rats, the model provides a good quantitative description not only of the first two moments (mean and variance) of the number of O-2A progenitor cells and oligodendrocytes at different times after the start of experiment, but of the corresponding distributions as well. As our estimates show, there are scarcely any O-2A progenitor cells that divide in vitro more than twice before they acquire the competence for differentiation. Those O-2A cells that have undergone the critical divisions differentiate into an oligodendrocyte in each of the subsequent mitotic cycles with a certain probability. We give estimates of this probability for O-2A cells under different growth conditions. Our analysis suggests that the effect of thyroid hormone is twofold: it reduces the mean duration of the mitotic cycle for progenitor cells, and it increases the probability of their transformation into oligodendrocytes. |
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Authors:
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K Boucher; A Y Yakovlev; M Mayer-Proschel; M Noble |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Mathematical biosciences Volume: 159 ISSN: 0025-5564 ISO Abbreviation: Math Biosci Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-07-01 Completed Date: 1999-07-01 Revised Date: 2009-11-11 |
Medline Journal Info:
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Nlm Unique ID: 0103146 Medline TA: Math Biosci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 47-78 Citation Subset: IM |
Affiliation:
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Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City 84108, USA. kenneth.boucher@hci.utah.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques Cell Cycle Cell Differentiation / physiology Computer Simulation Models, Biological* Numerical Analysis, Computer-Assisted Oligodendroglia / cytology* Optic Nerve / cytology Rats Stem Cells / cytology* Stochastic Processes Thyroid Hormones / physiology Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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5P30 CA42014/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Thyroid Hormones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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