Document Detail


A stochastic model of temporally regulated generation of oligodendrocytes in cell culture.
MedLine Citation:
PMID:  10361805     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The results of our previous analyses suggest that O-2A progenitor cells become competent for differentiation in vitro after they complete a certain number of critical mitotic cycles. The number of critical cycles varies from clone to clone and should be thought of as a random variable. We propose an approach to the analysis of oligodendrocyte generation in vitro based on a stochastic model allowing for an arbitrary distribution of this random variable with a finite support. When applied to experimental data on clonal growth and differentiation of purified O-2A progenitor cells obtained from optic nerves of 1 and 7 day-old rats, the model provides a good quantitative description not only of the first two moments (mean and variance) of the number of O-2A progenitor cells and oligodendrocytes at different times after the start of experiment, but of the corresponding distributions as well. As our estimates show, there are scarcely any O-2A progenitor cells that divide in vitro more than twice before they acquire the competence for differentiation. Those O-2A cells that have undergone the critical divisions differentiate into an oligodendrocyte in each of the subsequent mitotic cycles with a certain probability. We give estimates of this probability for O-2A cells under different growth conditions. Our analysis suggests that the effect of thyroid hormone is twofold: it reduces the mean duration of the mitotic cycle for progenitor cells, and it increases the probability of their transformation into oligodendrocytes.
Authors:
K Boucher; A Y Yakovlev; M Mayer-Proschel; M Noble
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mathematical biosciences     Volume:  159     ISSN:  0025-5564     ISO Abbreviation:  Math Biosci     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-07-01     Completed Date:  1999-07-01     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0103146     Medline TA:  Math Biosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  47-78     Citation Subset:  IM    
Affiliation:
Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City 84108, USA. kenneth.boucher@hci.utah.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Cycle
Cell Differentiation / physiology
Computer Simulation
Models, Biological*
Numerical Analysis, Computer-Assisted
Oligodendroglia / cytology*
Optic Nerve / cytology
Rats
Stem Cells / cytology*
Stochastic Processes
Thyroid Hormones / physiology
Time Factors
Grant Support
ID/Acronym/Agency:
5P30 CA42014/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Thyroid Hormones

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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