| A stochastic model of nonenzymatic nucleic acid replication: "elongators" sequester replicators. | |
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MedLine Citation:
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PMID: 17437149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The origin of nucleic acid template replication is a major unsolved problem in science. A novel stochastic model of nucleic acid chemistry was developed to allow rapid prototyping of chemical experiments designed to discover sufficient conditions for template replication. Experiments using the model brought to attention a robust property of nucleic acid template populations, the tendency for elongation to outcompete replication. Externally imposed denaturation-renaturation cycles did not reverse this tendency. For example, it has been proposed that fast tidal cycling could establish a TCR (tidal chain reaction) analogous to a PCR (polymerase chain reaction) acting on nucleic acid polymers, allowing their self-replication. However, elongating side-reactions that would have been prevented by the polymerase in the PCR still occurred in the simulation of the TCR. The same finding was found with temperature and monomer cycles. We propose that if cycling reactors are to allow template replication, oligonucleotide phenotypes that are capable of favorably altering the flux ratio between replication and elongation, for example, by facilitating sequence-specific cleavage within templates, are necessary; accordingly the minimal replicase ribozyme may have possessed restriction functionality. |
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Authors:
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Chrisantha Fernando; Günter Von Kiedrowski; Eörs Szathmáry |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-04-13 |
Journal Detail:
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Title: Journal of molecular evolution Volume: 64 ISSN: 0022-2844 ISO Abbreviation: J. Mol. Evol. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-05-07 Completed Date: 2007-08-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0360051 Medline TA: J Mol Evol Country: Germany |
Other Details:
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Languages: eng Pagination: 572-85 Citation Subset: IM |
Affiliation:
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School of Computer Science, University of Birmingham, Edgbaston, B15 2TT, UK. C.T.Fernando@cs.bham.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Enzymes Models, Chemical* Models, Molecular Nucleic Acid Conformation Nucleic Acid Denaturation Nucleic Acid Renaturation Nucleic Acids / chemical synthesis*, chemistry* Stochastic Processes Templates, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Enzymes; 0/Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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