Document Detail


Polydimethylsiloxane/polypyrrole stir bar sorptive extraction and liquid chromatography (SBSE/LC-UV) analysis of antidepressants in plasma samples.
MedLine Citation:
PMID:  19110116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new polymeric coating consisting of a dual-phase, polydimethylsiloxane (PDMS) and polypyrrole (PPY) was developed for the stir bar sorptive extraction (SBSE) of antidepressants (mirtazapine, citalopram, paroxetine, duloxetine, fluoxetine and sertraline) from plasma samples, followed by liquid chromatography analysis (SBSE/LC-UV). The extractions were based on both adsorption (PPY) and sorption (PDMS) mechanisms. SBSE variables, such as extraction time, temperature, pH of the matrix, and desorption time were optimized, in order to achieve suitable analytical sensitivity in a short time period. The PDMS/PPY coated stir bar showed high extraction efficiency (sensitivity and selectivity) toward the target analytes. The quantification limits (LOQ) of the SBSE/LC-UV method ranged from 20 ng mL(-1) to 50 ng mL(-1), and the linear range was from LOQ to 500 ng mL(-1), with a determination coefficient higher than 0.99. The inter-day precision of the SBSE/LC-UV method presented a variation coefficient lower than 15%. The efficiency of the SBSE/LC-UV method was proved by analysis of plasma samples from elderly depressed patients.
Authors:
L P Melo; A M Nogueira; F M Lanças; M E C Queiroz
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-25
Journal Detail:
Title:  Analytica chimica acta     Volume:  633     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2008-12-26     Completed Date:  2009-01-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  57-64     Citation Subset:  IM    
Affiliation:
Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Antidepressive Agents / blood*
Chromatography, Liquid / methods
Dimethylpolysiloxanes / chemical synthesis*
Hydrogen-Ion Concentration
Polymers / chemical synthesis*
Pyrroles / chemical synthesis*
Reproducibility of Results
Solid Phase Extraction / methods*
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Antidepressive Agents; 0/Dimethylpolysiloxanes; 0/Polymers; 0/Pyrroles; 30604-81-0/polypyrrole; 63148-62-9/baysilon

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