Document Detail


On the stimulation of viral DNA polymerase activity by nonionic detergent.
MedLine Citation:
PMID:  46756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nonionic detergents stimulate purified RNA-directed DNA polymerase (reverse transcriptase) activity from various RNA tumor viruses ranging from avian to primate species. The stimulatory effect of the nonionic detergent is dependent on the type and amount of template-primer. The greatest stimulation is obtained when high concentrations of (dT)12-18-(rA)n or activated salmon sperm DNA are used as template-primers. Little stimulation is obtained with viral 70S RNA or with (dT)12-18- (dA)n. The detergent stimulation appears to be specific for viral reverse transcriptase since this effect is not observed with purified bacterial DNA polymerase or with three known mammalian cellular DNA polymerases. This finding may, therefore, be a useful additional criterion for distinguishing viral reverse transcriptase isolated from cells from other cellular DNA polymerases. Nonionic detergent also has a stabilizing effect on viral DNA polymerase against thermal inactivation. This stabilizing effect is further enhanced by the presence of template-primer.
Authors:
A M Wu; A Cetta
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  14     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1975 Feb 
Date Detail:
Created Date:  1975-06-09     Completed Date:  1975-06-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  789-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
DNA Nucleotidyltransferases / metabolism*
Enzyme Activation / drug effects
Kinetics
Osmolar Concentration
Polyethylene Glycols / pharmacology
Polysorbates / pharmacology
Potassium Chloride / pharmacology
RNA Viruses / enzymology*
RNA-Directed DNA Polymerase / metabolism
Species Specificity
Surface-Active Agents / pharmacology*
Templates, Genetic
Time Factors
Chemical
Reg. No./Substance:
0/Polyethylene Glycols; 0/Polysorbates; 0/Surface-Active Agents; 7447-40-7/Potassium Chloride; EC 2.7.7.-/DNA Nucleotidyltransferases; EC 2.7.7.49/RNA-Directed DNA Polymerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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