Document Detail

PU/PTFE-stimulated monocyte-derived soluble factors induced inflammatory activation in endothelial cells.
MedLine Citation:
PMID:  19895882     Owner:  NLM     Status:  MEDLINE    
Polyurethane (PU) and polytetrafluoroethylene (PTFE) are two commonly used blood-contacting biomaterials. In the present study, we used a noncontact coculture model to evaluate the thrombosis-causing potential of monocyte-mediated PU and PTFE. We used human endothelial cells from umbilical cord (HUVECs) and human monocytes (THP1 cells). The THP1 cells were directly exposed to PU/PTFE, and the resultant cell-free supernatants were harvested for stimulating HUVECs. The treated HUVECs constituted the test group. HUVECs treated with supernatants of LPS-stimulated THP1 cells were used as the positive controls. To investigate the effects of the supernatant treatment on HUVECs, we measured the expression of the leukocyte-endothelial-cell adhesion molecules (CAMs) CD54 (ICAM-1), CD106 (VCAM-1), and CD62E (E-selectin) and evaluated the release of tissue factor (TF). The results demonstrated that both PU and PTFE induced the expressions of CD62E and TF. These activation effects were accompanied by activation of the NF-kappaB transcription factor. To further investigate the monocyte-derived soluble factors that might contribute to these effects, we evaluated the effects of the PU/PTFE stimulation on the expression of reactive oxygen species (ROS), TNF-alpha, IL-1beta, and IL-6 in monocyte monocultures. In comparison with the results for the negative control, both PU and PTFE significantly induced ROS release after 0.5h, while the expressions of TNF-alpha, IL-1beta, and IL-6 were variably increased after 24h. Our results suggest that the biomaterial induces monocytic activation and subsequently causes the release of soluble factors, which contribute to the inflammatory activation in HUVECs.
Yang Xue; Xin Liu; Jiao Sun
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-04
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  24     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  404-10     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Elsevier Ltd. All rights reserved.
Shanghai Biomaterials Research and Testing Center, Shanghai 200023, PR China.
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MeSH Terms
Cell Line
Coculture Techniques
Cytokines / metabolism
Endothelial Cells / drug effects*
Gene Expression Regulation / drug effects
Inflammation / chemically induced*
Monocytes / drug effects*
NF-kappa B / metabolism
Polytetrafluoroethylene / toxicity*
Polyurethanes / toxicity*
Reactive Oxygen Species
Reg. No./Substance:
0/Cytokines; 0/NF-kappa B; 0/Polyurethanes; 0/Reactive Oxygen Species; 9002-84-0/Polytetrafluoroethylene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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