Document Detail


Lutropin/choriogonadotropin stimulate the proliferation of primary cultures of rat Leydig cells through a pathway that involves activation of the extracellularly regulated kinase 1/2 cascade.
MedLine Citation:
PMID:  17412805     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Primary cultures of progenitor and immature rat Leydig cells were established from the testes of 21- and 35-d-old rats, respectively. The cell population remained homogeneous after 4-6 d in culture as judged by staining for 3beta-hydroxysteroid dehydrogenase, but the cells were unable to bind 125I-human chorionic gonadotropin (hCG) or to respond to hCG with classical LH receptor (LHR)-mediated responses, including cAMP and inositol phosphate accumulation, steroid biosynthesis, or the phosphorylation of ERK1/2. Infection of primary cultures with recombinant adenovirus coding for beta-galactosidase showed that approximately 65% of the cells are infected. Infection with adenovirus coding for the human LHR (hLHR) allowed for expression of the hLHR at a density of approximately 25,000 receptors per cell and allowed the cells to respond to hCG with increases in cAMP and inositol phosphate accumulation, steroid biosynthesis, and the phosphorylation of ERK1/2. Although progenitor and immature cells were able to respond to hCG with an increase in progesterone, only the immature cells responded with an increase in testosterone. In addition to these classical LHR-mediated responses, the primary cultures of progenitor or immature rat Leydig cells expressing the recombinant hLHR proliferated robustly when incubated with hCG, and this proliferative response was sensitive to an inhibitor of ERK1/2 phosphorylation. These studies establish a novel experimental paradigm that can be used to study the proliferative response of Leydig cells to LH/CG. We conclude that activation of the LHR-provoked Leydig cell proliferation requires activation of the ERK1/2 cascade.
Authors:
Koji Shiraishi; Mario Ascoli
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-04-05
Journal Detail:
Title:  Endocrinology     Volume:  148     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-18     Completed Date:  2007-10-02     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3214-25     Citation Subset:  AIM; IM    
Affiliation:
Department of Pharmacology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Butadienes / pharmacology
Cell Proliferation / drug effects*
Cells, Cultured
Chorionic Gonadotropin / pharmacology*
Cyclic AMP / metabolism
Dipeptides / pharmacology
Epidermal Growth Factor / pharmacology
Flavonoids / pharmacology
Immunohistochemistry
Inositol Phosphates / metabolism
Leydig Cells / cytology,  drug effects*,  metabolism
Luteinizing Hormone / pharmacology*
Male
Mitogen-Activated Protein Kinase 3 / metabolism*
Nitriles / pharmacology
Phosphorylation / drug effects
Pyrazoles / pharmacology
Pyrimidines / pharmacology
Quinazolines
Rats
Recombinant Proteins / metabolism
Signal Transduction / drug effects
Steroids / metabolism
Tyrphostins / pharmacology
Grant Support
ID/Acronym/Agency:
CA-40629/CA/NCI NIH HHS; R01 CA040629-23/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/4-amino-7-phenylpyrazol(3,4-d)pyrimidine; 0/AG 1879; 0/Butadienes; 0/Chorionic Gonadotropin; 0/Dipeptides; 0/Flavonoids; 0/Inositol Phosphates; 0/N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide; 0/Nitriles; 0/Pyrazoles; 0/Pyrimidines; 0/Quinazolines; 0/Recombinant Proteins; 0/Steroids; 0/Tyrphostins; 0/U 0126; 170449-18-0/tyrphostin AG 1478; 60-92-4/Cyclic AMP; 62229-50-9/Epidermal Growth Factor; 9002-67-9/Luteinizing Hormone; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3
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