Document Detail

sti35, a stress-responsive gene in Fusarium spp.
MedLine Citation:
PMID:  2376567     Owner:  NLM     Status:  MEDLINE    
A stress-induced mRNA was identified in the phytopathogenic fungus Fusarium oxysporum f. sp. cucumerinum. Treatment of the fungus with ethanol resulted in the induction of a major mRNA species encoding a protein of approximate Mr 37,000. A full-length cDNA clone of the induced message was obtained. RNA blot analysis indicated that the mRNA was induced by various other stresses, including treatment with copper(II) chloride and heat (37 degrees C). However, it was not greatly induced by treatment with phaseollinisoflavan, an antifungal isoflavonoid produced by Phaseolus vulgaris (French bean). In contrast, phaseollinisoflavan induced the homologous mRNA in the related bean pathogen Fusarium solani f. sp. phaseoli. A genomic clone of the F. solani f. sp. phaseoli gene was obtained, and both this and the cDNA clone from F. oxysporum f. sp. cucumerinum were sequenced. The latter indicated an open reading frame of 320 codons encoding a 34,556-dalton polypeptide. The corresponding reading frame in F. solani f. sp. phaseoli was 324 codons, 89% identical to the F. oxysporum f. sp. cucumerium sequence, and was interrupted by a short intron. The gene was designated sti35 (stress-inducible mRNA). Although computer homology searches were negative, the cloned gene was observed to cross-hybridize to DNAs of other filamentous fungi, Saccharomyces cerevisiae, and soybean. Thus, sti35 appears to be a common gene among a variety of eucaryotes.
G H Choi; E T Marek; C L Schardl; M G Richey; S Y Chang; D A Smith
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  172     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-08-31     Completed Date:  1990-08-31     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4522-8     Citation Subset:  IM    
Department of Plant Pathology, University of Kentucky, Lexington 40546.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M33642;  M33643
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Codon / genetics
Copper / pharmacology
DNA Probes
DNA, Fungal / genetics,  isolation & purification
Ethanol / pharmacology
Fusarium / drug effects,  genetics*,  physiology
Gene Library
Genes, Fungal* / drug effects
Genetic Vectors
Hot Temperature
Molecular Sequence Data
Nucleic Acid Hybridization
Protein Biosynthesis
RNA, Fungal / genetics,  isolation & purification
RNA, Messenger / genetics,  isolation & purification
Grant Support
1 U 41 RR-01685/RR/NCRR NIH HHS
Reg. No./Substance:
0/Codon; 0/DNA Probes; 0/DNA, Fungal; 0/RNA, Fungal; 0/RNA, Messenger; 64-17-5/Ethanol; 7440-50-8/Copper

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