| A steroidogenic cell line with differentiation potential from mouse granulosa cells, transfected with Ad4BP and SV40 large T antigen genes. | |
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MedLine Citation:
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PMID: 15817839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several steroidogenic cell lines of granulosa cells (GC) have been used to elucidate differentiation mechanisms of GC during folliculogenesis. These cell lines, however, are of limited usefulness since they have lost some of their differentiation potential. The transcription factor adrenal-4 binding protein (Ad4BP), also known as steroidogenic factor-1 or NR5A1, is essential for the expression of all P-450 steroidogenic enzymes. By transfection with the Ad4BP gene together with SV40 DNA, we have generated several steroidogenic cell lines. One selective clone, named 4B2, retained its steroidogenic potential and was therefore analyzed in depth. This cell line responded to 8-Br-cAMP by displaying differentiation characteristics similar to those occurring in the differentiation process of primary cultured GC, including enhanced progesterone secretion, a cell shape change from a fibroblastic to epithelioid conformation, elongated mitochondria, increased gap junction formation and inhibition of cell proliferation. Prostaglandin E2 (PGE2), an intraovarian regulator of GC, stimulated cAMP production, and this eicosanoid, like 8-Br-cAMP, induced differentiation properties with the exception of cell conformation in 4B2 cells. These results suggest that expression of Ad4BP may provide the basis for a repertoire of cAMP-sensitive differentiation properties, including morphological alterations and growth inhibition. Thus, the 4B2 cell line may serve as a tool for elucidation of differentiation mechanisms that are under the control of Ad4BP. |
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Authors:
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Y Kamei; Y Aoyama; T Fujimoto; N Kenmotsu; C Kishi; M Koushi; S Sugano; K Morohashi; R Kamiyama; R Asakai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of endocrinology Volume: 185 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-08 Completed Date: 2005-05-17 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 187-95 Citation Subset: IM |
Affiliation:
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Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima Bunkyouku, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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8-Bromo Cyclic Adenosine Monophosphate
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pharmacology Animals Antigens, Polyomavirus Transforming / analysis, genetics*, metabolism Cell Differentiation Cell Line Cyclic AMP / metabolism DNA-Binding Proteins / analysis, genetics*, metabolism Dinoprostone / pharmacology Female Gap Junctions / ultrastructure Granulosa Cells / cytology, metabolism*, ultrastructure Homeodomain Proteins Immunohistochemistry / methods Mice Mitochondria / ultrastructure Progesterone / analysis Radioimmunoassay / methods Receptors, Cytoplasmic and Nuclear Steroidogenic Factor 1 Steroids / biosynthesis* Transcription Factors / analysis, genetics*, metabolism Transfection / methods |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Polyomavirus Transforming; 0/DNA-Binding Proteins; 0/Homeodomain Proteins; 0/Receptors, Cytoplasmic and Nuclear; 0/Steroidogenic Factor 1; 0/Steroids; 0/Transcription Factors; 0/steroidogenic factor 1, mouse; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 363-24-6/Dinoprostone; 57-83-0/Progesterone; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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