Document Detail

The steady-state amino acid fluxes across the perfused choroid plexus of the sheep.
MedLine Citation:
PMID:  2123729     Owner:  NLM     Status:  MEDLINE    
The steady-state flux of labelled amino acids was investigated across the isolated perfused choroid plexus of the sheep. The extraction of anionic, cationic, small and large neutral amino acids by the blood side of the choroid plexus was demonstrated. However, there was no uptake of the analogue MeAIB, confirming the absence of the 'A' carrier system on this side of the blood--CSF barrier. The direction of the net flux of amino acids across the tissue varied depending on the amino acid and its concentration. At a concentration of 0.01 mM the net movement for phenylalanine, serine, aspartate and glycine was from blood to CSF. When the concentration of amino acid was increased to 0.1 mM, the net flux of phenylalanine and serine remained from blood to CSF whereas the net flux of the transmitters, aspartate and glycine, was in the opposite direction, from CSF to blood. When the level was raised further, to 1 mM, all four amino acids showed a net CSF to blood flux. The concentration of amino acids is newly formed CSF was calculated from the blood to CSF fluxes and was found to be between 2 and 200 microM, similar to that found in mammalian bulk phase CSF.
J E Preston; M B Segal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  525     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1991-01-24     Completed Date:  1991-01-24     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  275-9     Citation Subset:  IM    
Sherrington School of Physiology, Hospital, London, U.K.
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MeSH Terms
Amino Acids / blood,  cerebrospinal fluid,  metabolism*
Biological Transport
Carbon Radioisotopes
Choroid Plexus / metabolism*
Mannitol / metabolism
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Amino Acids; 0/Carbon Radioisotopes; 10028-17-8/Tritium; 69-65-8/Mannitol

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