| The steady-state amino acid fluxes across the perfused choroid plexus of the sheep. | |
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MedLine Citation:
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PMID: 2123729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The steady-state flux of labelled amino acids was investigated across the isolated perfused choroid plexus of the sheep. The extraction of anionic, cationic, small and large neutral amino acids by the blood side of the choroid plexus was demonstrated. However, there was no uptake of the analogue MeAIB, confirming the absence of the 'A' carrier system on this side of the blood--CSF barrier. The direction of the net flux of amino acids across the tissue varied depending on the amino acid and its concentration. At a concentration of 0.01 mM the net movement for phenylalanine, serine, aspartate and glycine was from blood to CSF. When the concentration of amino acid was increased to 0.1 mM, the net flux of phenylalanine and serine remained from blood to CSF whereas the net flux of the transmitters, aspartate and glycine, was in the opposite direction, from CSF to blood. When the level was raised further, to 1 mM, all four amino acids showed a net CSF to blood flux. The concentration of amino acids is newly formed CSF was calculated from the blood to CSF fluxes and was found to be between 2 and 200 microM, similar to that found in mammalian bulk phase CSF. |
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Authors:
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J E Preston; M B Segal |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain research Volume: 525 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1991-01-24 Completed Date: 1991-01-24 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 275-9 Citation Subset: IM |
Affiliation:
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Sherrington School of Physiology, Hospital, London, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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blood,
cerebrospinal fluid,
metabolism* Animals Biological Transport Carbon Radioisotopes Choroid Plexus / metabolism* Kinetics Mannitol / metabolism Perfusion Sheep Tritium |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbon Radioisotopes; 10028-17-8/Tritium; 69-65-8/Mannitol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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