Document Detail


A statistical method for analysis of mouse lymphoma L5178Y cell TK locus forward mutation assay. Comparison of results among three laboratories.
MedLine Citation:
PMID:  7144804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Analysis of data from our laboratory and that of two National Cancer Institute contractor laboratories indicate the random variation in the results of the mouse lymphoma L5178Y cell TK locus mutation assay can be adequately described by a lognormal distribution. This indicates that transformation of mutant frequencies to logarithms enables one to properly use well-known statistical techniques such as analysis of variance, regression analysis, and Student's t-test for the interpretation of data from this assay. The consistency of the lognormal distribution among laboratories is demonstrated. Three examples which illustrate the mechanics and interpretation of the proposed methodology are included. It is concluded that the method is effective in identifying weak mutagens as well as enabling the user to compute the uncertainty associated with an observed increase in mutagenic activity.
Authors:
J D Irr; R D Snee
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  97     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1983-01-07     Completed Date:  1983-01-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  371-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Dose-Response Relationship, Drug
Laboratories
Leukemia L5178 / enzymology,  genetics*
Leukemia, Experimental / genetics*
Mice
Mutagenicity Tests*
Mutagens / pharmacology
Statistics as Topic*
Thymidine Kinase / genetics*
Chemical
Reg. No./Substance:
0/Mutagens; EC 2.7.1.21/Thymidine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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