| The state of aggregation of red deer (Cervus elaphus L.) beta-lactoglobulin preparations near neutral pH. | |
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MedLine Citation:
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PMID: 22326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The state of aggregation of four red-deer (Cervus elaphus L.) beta-lactoglobulin preparations and a control ox beta-lactoglobulin A preparation was studied by sedimentation-equilibrium experiments at pH 6.5 and 20 degrees C. 2. Three of the deer preparations and the ox control each behaved as a monomer-dimer system, with a value of log K (where K is the association constant in litres/mol) in the range 5.4-5.5. 3. When one of these deer preparations was examined in the presence of dithiothreitol, log K appeared to decrease to 4.5.4. One deer preparation, comprising recovered material, appeared to have undergone irreversible changes and to behave like a non-equilibrating system containing monomer, dimer and trimer. 5. The sedimentation-equilibrium properties of the deer monomer was studied in 6M-guanidine hydrochloride at pH 7.0; the mol.wt. was 17600, the second virial coefficient was 3.4 x 10(-3) ml - mol - g-2, and the apparent partial specific volume 0.724 ml/g, a value indicating an appreciable decrease in volume on dissociation and denaturation. |
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Authors:
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E I McDougall; J C Stewart |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 167 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1977 Oct |
Date Detail:
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Created Date: 1978-01-27 Completed Date: 1978-01-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 45-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Deer / metabolism* Female Guanidines Hydrogen-Ion Concentration Lactoglobulins / metabolism* Macromolecular Substances Molecular Weight |
| Chemical | |
Reg. No./Substance:
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0/Guanidines; 0/Lactoglobulins; 0/Macromolecular Substances |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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