Document Detail

The staphylococcal pore-forming leukotoxins open Ca2+ channels in the membrane of human polymorphonuclear neutrophils.
MedLine Citation:
PMID:  9543493     Owner:  NLM     Status:  MEDLINE    
The ability of leukotoxins secreted by Staphylococcus aureus to modify the permeability of the membrane of human polymorphonuclear neutrophils has been studied by spectrofluorometry and appropriate fluorescent probes. This family of bicomponent leukotoxins is constituted by, at least, three pairs of proteins: LukS-PV/LukF-PV, HlgA/HlgB, HlgC/HlgB. After binding of both components to the membrane, each pair induces influxes of divalent cations and ethidium in polymorphonuclear neutrophils, although with different intensities. The influx of divalent cations appears sooner than the influx of ethidium. The pathway for divalent cations is not permeable to monovalent cations (Na+, K+, ethidium+) and is blocked by Ca2+ channel inhibitors that do not block the fluxes of ethidium and monovalent cations. It is concluded that the leukotoxins bind to a receptor linked to a divalent cation-selective channel or to the channel itself which is activated. Then, the leukotoxins open a second pathway by insertion into the membrane and subsequent formation of aspecific pores allowing an influx of ethidium.
L Staali; H Monteil; D A Colin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of membrane biology     Volume:  162     ISSN:  0022-2631     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-14     Completed Date:  1998-05-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  209-16     Citation Subset:  IM    
Laboratoire de Toxinologie et d'Antibiologie Bactériennes, Institut de Bactériologie, Université Louis Pasteur, Strasbourg, France.
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MeSH Terms
Bacterial Proteins*
Bacterial Toxins / pharmacology*
Calcium Channel Blockers / pharmacology
Calcium Channels / drug effects*,  physiology
Cell Membrane / drug effects
Hemolysin Proteins / pharmacology*
Leukocidins / pharmacology*
Neutrophils / drug effects*,  physiology
Staphylococcus aureus / metabolism*
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacterial Toxins; 0/Calcium Channel Blockers; 0/Calcium Channels; 0/Cations; 0/Hemolysin Proteins; 0/Leukocidins; 0/gamma-hemolysin, Staphylococcus aureus; 0/leukocidin S-component protein, Staphylococcus; 147096-70-6/LukF protein, Staphylococcus aureus

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