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PSAQ™ standards for accurate MS-based quantification of proteins: from the concept to biomedical applications.
MedLine Citation:
PMID:  23019168     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Absolute protein quantification, i.e. determining protein concentrations in biological samples, is essential to our understanding of biological and physiopathological phenomena. Protein quantification methods based on the use of antibodies are very effective and widely used. However, over the last ten years, absolute protein quantification by mass spectrometry has attracted considerable interest, particularly for the study of systems biology and as part of biomarker development. This interest is mainly linked to the high multiplexing capacity of MS analysis, and to the availability of stable-isotope-labelled standards for quantification. This article describes the details of how to produce, control the quality and use a specific type of standard: Protein Standard Absolute Quantification (PSAQ™) standards. These standards are whole isotopically labelled proteins, analogues of the proteins to be assayed. PSAQ standards can be added early during sample treatment, thus they can correct for protein losses during sample prefractionation and for incomplete sample digestion. Because of this, quantification of target proteins is very accurate and precise using these standards. To illustrate the advantages of the PSAQ method, and to contribute to the increase in its use, selected applications in the biomedical field are detailed here. Copyright © 2012 John Wiley & Sons, Ltd.
Authors:
Guillaume Picard; Dorothée Lebert; Mathilde Louwagie; Annie Adrait; Céline Huillet; François Vandenesch; Christophe Bruley; Jérôme Garin; Michel Jaquinod; Virginie Brun
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of mass spectrometry : JMS     Volume:  47     ISSN:  1096-9888     ISO Abbreviation:  J Mass Spectrom     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504818     Medline TA:  J Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1353-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Affiliation:
CEA, IRTSV, Biologie à Grande Echelle, F-38054, Grenoble, France; INSERM, U1038, F-38054, Grenoble, France; Université Joseph Fourier, Grenoble 1, F-38000, France; Promise Advanced Proteomics, Grenoble, F-38040, France.
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