| The staircase phenomenon and the strength-interval relationship of contractions in cultured cells of chicken embryonic ventricles. | |
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MedLine Citation:
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PMID: 8007445 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The staircase phenomenon and the relationship between contraction strength and stimulation interval were investigated in single or aggregated cells cultured from 5-, 12-, and 19-d-old chicken embryonic ventricles. Within 24 h after start of cultivation was begun, the cells were separated and spontaneously inactive. However, they contracted when electrical stimulation was applied. The modes of a staircase phenomenon and a strength-interval relationship varied, depending on a few conditions such as the ages of the embryos, the presence of spontaneous activities and the composition of the bathing solution. The 19-d-old cells, which formed an aggregate and exhibited spontaneous activities, showed a negative staircase phenomenon and the negative strength-interval relationship when they were bathed in a culture medium containing bovine serum. The 12-d-old cells demonstrated a less distinct staircase and a flat strength-interval relationship. Both 19- and 12-d-old cells showed a positive relationship, if they were cultured for less than 48 h and were spontaneously inactive, or if they were bathed in Tyrode solution during the experiments. The 5-d-old cells showed the positive staircase whether they were spontaneously active or not. The developmental change in the interval-dependency of contraction can be explained by the functions of the sarcoplasmic reticulum (SR), although Ca2+ current and the Na(+)-Ca2+ exchange mechanism should be involved. The function of the SR develops progressively during the embryonic stage and plays an important role in regulation of the contraction-relaxation cycle at the late stage before hatching. |
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Authors:
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H Toyota; M Matsumura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Japanese journal of physiology Volume: 43 ISSN: 0021-521X ISO Abbreviation: Jpn. J. Physiol. Publication Date: 1993 |
Date Detail:
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Created Date: 1994-07-20 Completed Date: 1994-07-20 Revised Date: 2007-03-21 |
Medline Journal Info:
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Nlm Unique ID: 2985184R Medline TA: Jpn J Physiol Country: JAPAN |
Other Details:
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Languages: eng Pagination: 759-74 Citation Subset: IM |
Affiliation:
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Department of Physiology, Kawasaki Medical School, Kurashiki, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division Chick Embryo Heart / embryology* Heart Ventricles Myocardial Contraction* Myocardium / cytology |
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