| The stable o-phthalaldehyde-ferrocene/6-ferrocenyl-1-hexanethiol pre-column derivatization high-performance liquid chromatography of dimethylarginine by novel dual fluorescence and electrochemical detector. | |
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MedLine Citation:
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PMID: 23018892 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Monomethylarginine, asymmetric dimethylarginine and symmetric dimethylarginine were separated on a Wakopak Combi ODS with an acetonitrile-100 mm potassium phosphate buffer (pH 7.0; 1:1, v/v). Dimethylarginines were derived from o-phthalaldehyde for the fluorescence detector and from 6-ferrocenyl-1-hexanethiol for the electrochemical detector. The detection limits of the dimethylarginines in spiked plasma were 0.3-0.5 pmol by electrochemical detection and 1-2 pmol by fluorescence detection. The detection limits were improved over 30 times by electrochemical detection and 10 times by fluorescence detection compared with previous reports. In previous derivatization liquid chromatography, the reaction solutions, o-phthalaldehyde, 2-mercaptethanol and dimethylarginines were unstable and required quick derivatization at 4°C. By our proposed pre-column methods, the dimethylarginines were derivatized at room temperature and the fluorescent products were stable for 6 h. The manipulation performance was greatly advanced compared with previous LC reports. This is the first report on stable and sensitive dimethylarginines by dual detection. The selectivity was also improved by dual detection. The proposed method was applied to preliminary monitoring of dimethylargines in plasma and urine. Copyright © 2012 John Wiley & Sons, Ltd. |
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Authors:
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Katsuhiro Suzuki; Masatoki Katayama; Kiyoshi Takamatsu; Satoru Kaneko; Hiromichi Ishikawa; Takahiro Tsukimura; Tadayasu Togawa |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-28 |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: - ISSN: 1099-0801 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 John Wiley & Sons, Ltd. |
Affiliation:
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Drug Metabolism Research Laboratories, Drug Discovery Research, Astellas Pharma Inc., Kashima, Yodogawa-ku, Osaka, 532-8514, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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