Document Detail


A stable and selective electrochemical biosensor for the liver enzyme alanine aminotransferase (ALT).
MedLine Citation:
PMID:  19356918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An electrochemical method to determine alanine aminotransferase (ALT) activity over its normal and elevated physiological range was developed based upon detection of L-glutamate at a glutamate oxidase-modified platinum electrode. Measurements were carried out in the presence of ALT co-substrates L-alanine and alpha-ketoglutarate and current response from either the oxidation of hydrogen peroxide or the re-oxidation of the mediator ferrocene carboxylic acid was employed. The enzyme electrode was tested over a 6-month period and found to retain 79% of its original activity towards ALT detection with >200 measurements performed over this time. Signals associated with interfering electroactive species (ascorbic acid and uric acid) were eliminated using background subtraction at a denatured glutamate oxidase enzyme electrode. The sensitivity of the device was found to be 0.845 nA U(-1) L ALT with t(90)=180 s, linear range 10-1000 U L(-1) and LOD of 3.29 U L(-1) using amperometry at E(app)=0.4 V vs. Ag/AgCl at 308 K (35 degrees C).
Authors:
Mamun Jamal; Oliver Worsfold; Timothy McCormac; Eithne Dempsey
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-11
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  24     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-20     Completed Date:  2009-07-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2926-30     Citation Subset:  IM    
Affiliation:
Centre for Research in Electroanalytical Technologies (CREATE), Department of Science, Institute of Technology Tallaght, Tallaght, Dublin 24, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Alanine / metabolism
Alanine Transaminase / analysis*,  metabolism
Amino Acid Oxidoreductases / metabolism*
Biosensing Techniques / economics,  methods*
Enzymes, Immobilized / metabolism
Ferrous Compounds / metabolism
Glutamic Acid / analysis*,  metabolism
Humans
Hydrogen Peroxide / metabolism
Ketoglutaric Acids / metabolism
Liver / enzymology
Oxidation-Reduction
Platinum / chemistry*
Sensitivity and Specificity
Time Factors
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Ferrous Compounds; 0/Ketoglutaric Acids; 1271-42-7/ferrocenecarboxylic acid; 328-50-7/alpha-ketoglutaric acid; 56-41-7/Alanine; 56-86-0/Glutamic Acid; 7440-06-4/Platinum; 7722-84-1/Hydrogen Peroxide; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.3.11/L-glutamate oxidase; EC 2.6.1.2/Alanine Transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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