Document Detail


A stable colorimetric assay to measure toxin elevation of inorganic phosphate in bile.
MedLine Citation:
PMID:  3364737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modified assay for the measurement of nanomole amounts of Pi in 50 microliters of bile is described. The assay is based on the formation of a complex between malachite green dye and phosphomolybdate under acidic conditions. Only three simple steps are required to produce a colored complex which remains stable for at least 3 h; these steps are precipitation with acid, addition of color reagent containing dye and surfactant, and incubation at 37 degrees C. Analysis of bile samples collected from a rat given the toxin 1,1-dichloroethylene demonstrated that the assay is well suited to routine assays of biliary Pi as an endogenous indicator of aberrant hepatobiliary function.
Authors:
M T Moslen; M F Kanz; A E Ferguson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  168     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-05-31     Completed Date:  1988-05-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  405-10     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Texas Medical Branch, Galveston.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile / analysis*
Dichloroethylenes / pharmacology
Phosphates / analysis*
Phospholipids / pharmacology
Rats
Rosaniline Dyes / diagnostic use
Spectrophotometry
Toxins, Biological / pharmacology*
Grant Support
ID/Acronym/Agency:
AM 34806/AM/NIADDK NIH HHS; ES 03368/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Dichloroethylenes; 0/Phosphates; 0/Phospholipids; 0/Rosaniline Dyes; 0/Toxins, Biological; 10309-95-2/malachite green

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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