| A stable colorimetric assay to measure toxin elevation of inorganic phosphate in bile. | |
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MedLine Citation:
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PMID: 3364737 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A modified assay for the measurement of nanomole amounts of Pi in 50 microliters of bile is described. The assay is based on the formation of a complex between malachite green dye and phosphomolybdate under acidic conditions. Only three simple steps are required to produce a colored complex which remains stable for at least 3 h; these steps are precipitation with acid, addition of color reagent containing dye and surfactant, and incubation at 37 degrees C. Analysis of bile samples collected from a rat given the toxin 1,1-dichloroethylene demonstrated that the assay is well suited to routine assays of biliary Pi as an endogenous indicator of aberrant hepatobiliary function. |
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Authors:
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M T Moslen; M F Kanz; A E Ferguson |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 168 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1988 Feb |
Date Detail:
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Created Date: 1988-05-31 Completed Date: 1988-05-31 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 405-10 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of Texas Medical Branch, Galveston. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile / analysis* Dichloroethylenes / pharmacology Phosphates / analysis* Phospholipids / pharmacology Rats Rosaniline Dyes / diagnostic use Spectrophotometry Toxins, Biological / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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AM 34806/AM/NIADDK NIH HHS; ES 03368/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Dichloroethylenes; 0/Phosphates; 0/Phospholipids; 0/Rosaniline Dyes; 0/Toxins, Biological; 10309-95-2/malachite green |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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