Document Detail


A squash technique for studying the cytology of maize endosperm and other tissues.
MedLine Citation:
PMID:  333656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new cytological procedure specifically suited to maize endosperms is presented. It uses 8-hydroxyquinoline with sucrose and aeration to pretreat the tissues. Glusulase is used to spread the cells. The procedure makes it possible to squash endosperms into a single cell layer and to photograph as many as 70 chromosomes in the same focal plane. It also allows identification of translocation chromosomes. With a slight modification the technique has been applied successfully to root tips and other tissues.
Authors:
B Y Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  52     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1977 Jul 
Date Detail:
Created Date:  1977-11-30     Completed Date:  1977-11-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  197-201     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosomes / ultrastructure*
Cytological Techniques*
Hydroxyquinolines / diagnostic use
Karyotyping
Translocation, Genetic
Zea mays / ultrastructure*
Chemical
Reg. No./Substance:
0/Hydroxyquinolines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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