| A squash technique for studying the cytology of maize endosperm and other tissues. | |
MedLine Citation:
|
PMID: 333656 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A new cytological procedure specifically suited to maize endosperms is presented. It uses 8-hydroxyquinoline with sucrose and aeration to pretreat the tissues. Glusulase is used to spread the cells. The procedure makes it possible to squash endosperms into a single cell layer and to photograph as many as 70 chromosomes in the same focal plane. It also allows identification of translocation chromosomes. With a slight modification the technique has been applied successfully to root tips and other tissues. |
Authors:
|
B Y Lin |
Related Documents
:
|
23526746 - Genome-wide linkage and regional association study of obesity-related phenotypes: the g... 541616 - The detection of y chromosomes in bloodstains--a reevaluation. 914276 - Evidence for postmeiotic expression of ribosomal rna genes during male gametogenesis. 3303456 - A quick enzyme squash technique for detailed studies on female meiosis in solanum. 1459426 - Sister-chromatid misbehavior in drosophila ord mutants. 12197146 - Allozyme markers for the identification of lecithochirium rufoviride and lecithochirium... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Stain technology Volume: 52 ISSN: 0038-9153 ISO Abbreviation: Stain Technol Publication Date: 1977 Jul |
Date Detail:
|
Created Date: 1977-11-30 Completed Date: 1977-11-30 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0404535 Medline TA: Stain Technol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 197-201 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromosomes
/
ultrastructure* Cytological Techniques* Hydroxyquinolines / diagnostic use Karyotyping Translocation, Genetic Zea mays / ultrastructure* |
| Chemical | |
Reg. No./Substance:
|
0/Hydroxyquinolines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ophthalmology in the United States at the time of the revolution.
Next Document: A specific enzymeimmunoassay for progesterone in human plasma.