Document Detail


The sporadic occurrence of a group I intron-like element in the mtDNA rnl gene of Ophiostoma novo-ulmi subsp. americana.
MedLine Citation:
PMID:  18406119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of group I intron-like elements within the U7 region of the mtDNA large ribosomal subunit RNA gene (rnl) was investigated in strains of Ophiostoma novo-ulmi subsp. americana from Canada, Europe and Eurasia, and in selected strains of O. ips, O. minus, O. piceae, O. ulmi, and O. himal-ulmi. This insertion is of interest as it has been linked previously to the generation of plasmid-like mtDNA elements in diseased strains of O. novo-ulmi. Among 197 O. novo-ulmi subsp. americana strains tested, 61 contained a 1.6kb insertion within the rnl-U7 region and DNA sequence analysis suggests the presence of a group I intron (IA1 type) that encodes a potential double motif LAGLIDADG homing endonuclease-like gene (HEG). Phylogenetic analysis of rnl-U7 intron encoded HEG-like elements supports the view that double motif HEGs originated from a duplication event of a single-motif HEG followed by a fusion event that combined the two copies into one open reading frame (ORF). The data also show that rnl-U7 intron encoded ORFs belong to a clade that includes ORFs inserted into different types of group I introns, e.g. IB, ID, IC3, IA1, present within a variety of different mtDNA genes, such as the small ribosomal subunit RNA gene (rns), apo-cytochrome b gene (cob), NADH dehydrogenase subunit 5 (nad5), cytochrome oxidase subunit 1 gene (coxI), and ATPase subunit 9 gene (atp9). We also compared the occurrence of the rnl-U7 intron in our collection of 227 strains with the presence of the rnl-U11 group I intron and concluded that the U7 intron appears to be an optional element and the U11 intron is probably essential among the strains tested.
Authors:
Jyothi Sethuraman; Chukwuemeka V Okoli; Anna Majer; Tamara L C Corkery; Georg Hausner
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-23
Journal Detail:
Title:  Mycological research     Volume:  112     ISSN:  0953-7562     ISO Abbreviation:  Mycol. Res.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-07-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8913481     Medline TA:  Mycol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  564-82     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Ascomycota / chemistry,  classification,  genetics*,  isolation & purification
Base Pairing
DNA, Fungal / chemistry,  genetics
DNA, Mitochondrial / chemistry,  genetics*
Fungal Proteins / chemistry,  genetics
Introns*
Mitochondria / chemistry,  genetics
Molecular Sequence Data
Mutagenesis, Insertional
Open Reading Frames
Phylogeny
Plant Diseases / microbiology
Sequence Alignment
Ulmus / microbiology
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Mitochondrial; 0/Fungal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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