| The sporadic occurrence of a group I intron-like element in the mtDNA rnl gene of Ophiostoma novo-ulmi subsp. americana. | |
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MedLine Citation:
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PMID: 18406119 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The presence of group I intron-like elements within the U7 region of the mtDNA large ribosomal subunit RNA gene (rnl) was investigated in strains of Ophiostoma novo-ulmi subsp. americana from Canada, Europe and Eurasia, and in selected strains of O. ips, O. minus, O. piceae, O. ulmi, and O. himal-ulmi. This insertion is of interest as it has been linked previously to the generation of plasmid-like mtDNA elements in diseased strains of O. novo-ulmi. Among 197 O. novo-ulmi subsp. americana strains tested, 61 contained a 1.6kb insertion within the rnl-U7 region and DNA sequence analysis suggests the presence of a group I intron (IA1 type) that encodes a potential double motif LAGLIDADG homing endonuclease-like gene (HEG). Phylogenetic analysis of rnl-U7 intron encoded HEG-like elements supports the view that double motif HEGs originated from a duplication event of a single-motif HEG followed by a fusion event that combined the two copies into one open reading frame (ORF). The data also show that rnl-U7 intron encoded ORFs belong to a clade that includes ORFs inserted into different types of group I introns, e.g. IB, ID, IC3, IA1, present within a variety of different mtDNA genes, such as the small ribosomal subunit RNA gene (rns), apo-cytochrome b gene (cob), NADH dehydrogenase subunit 5 (nad5), cytochrome oxidase subunit 1 gene (coxI), and ATPase subunit 9 gene (atp9). We also compared the occurrence of the rnl-U7 intron in our collection of 227 strains with the presence of the rnl-U11 group I intron and concluded that the U7 intron appears to be an optional element and the U11 intron is probably essential among the strains tested. |
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Authors:
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Jyothi Sethuraman; Chukwuemeka V Okoli; Anna Majer; Tamara L C Corkery; Georg Hausner |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-23 |
Journal Detail:
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Title: Mycological research Volume: 112 ISSN: 0953-7562 ISO Abbreviation: Mycol. Res. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-29 Completed Date: 2008-07-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8913481 Medline TA: Mycol Res Country: England |
Other Details:
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Languages: eng Pagination: 564-82 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Ascomycota / chemistry, classification, genetics*, isolation & purification Base Pairing DNA, Fungal / chemistry, genetics DNA, Mitochondrial / chemistry, genetics* Fungal Proteins / chemistry, genetics Introns* Mitochondria / chemistry, genetics Molecular Sequence Data Mutagenesis, Insertional Open Reading Frames Phylogeny Plant Diseases / microbiology Sequence Alignment Ulmus / microbiology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal; 0/DNA, Mitochondrial; 0/Fungal Proteins |
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