Document Detail


A spontaneous deletion of α-synuclein is associated with an increase in CB1 mRNA transcript and receptor expression in the hippocampus and amygdala: effects on alcohol consumption.
MedLine Citation:
PMID:  23345080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
α-Synuclein (α-syn) protein and endocannabinoid CB1 receptors are primarily located in presynaptic terminals. An association between α-syn and CB1 receptors has recently been established in Parkinson's disease, but it is completely unknown whether there is an association between these two proteins in alcohol addiction. Therefore, we aimed to examine the α-syn mRNA transcript and protein expression levels in the prefrontal cortex, striatum, amygdala and hippocampus. These brain regions are the most frequently implicated in alcohol and other drug addiction. In these studies, we used C57BL/6 mice carrying a spontaneous deletion of the α-syn gene (C57BL/6(Snca-/-) ) and their respective controls (C57BL/6(Snca) (+/) (+) ). These animals were monitored for spontaneous alcohol consumption (3-10%) and their response to a hypnotic-sedative dose of alcohol (3 g kg(-1) ) was also assessed. Compared with the C57BL/6(Snca+/+) mice, we found that the C57BL/6(Snca-/-) mice exhibited a higher expression level of the CB1 mRNA transcript and CB1 receptor in the hippocampus and amygdala. Furthermore, C57BL/6(Snca-/-) mice showed an increase in alcohol consumption when offered a 10% alcohol solution. There was no significant difference in sleep time after the injection of 3 g/kg alcohol. These results are the first to reveal an association between α-syn and the CB1 receptor in the brain regions that are most frequently implicated in alcohol and other drug addictions.
Authors:
Alejandro López-Jiménez; Nicole A R Walter; Elena Giné; Ángel Santos; Victor Echeverry-Alzate; Kora-Mareen Bühler; Pedro Olmos; Stéphanie Giezendanner; Rosario Moratalla; Lluis Montoliu; Kari J Buck; Jose Antonio López-Moreno
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-20
Journal Detail:
Title:  Synapse (New York, N.Y.)     Volume:  67     ISSN:  1098-2396     ISO Abbreviation:  Synapse     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-18     Completed Date:  2013-10-29     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  8806914     Medline TA:  Synapse     Country:  United States    
Other Details:
Languages:  eng     Pagination:  280-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Alcohol Drinking / genetics*
Amygdala / metabolism*,  physiology
Animals
Ethanol / pharmacology
Gene Deletion
Hippocampus / metabolism*,  physiology
Mice
Mice, Inbred C57BL
Prefrontal Cortex / metabolism,  physiology
RNA, Messenger / genetics,  metabolism
Receptor, Cannabinoid, CB1 / genetics,  metabolism*
Sleep / drug effects
Transcription, Genetic*
alpha-Synuclein / genetics*,  metabolism
Grant Support
ID/Acronym/Agency:
AA010760/AA/NIAAA NIH HHS; AA011114/AA/NIAAA NIH HHS; P50 AA010760/AA/NIAAA NIH HHS; R01 AA011114/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptor, Cannabinoid, CB1; 0/Snca protein, mouse; 0/alpha-Synuclein; 3K9958V90M/Ethanol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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