| The splicing regulator PTBP2 interacts with the cytidine deaminase AID and promotes binding of AID to switch-region DNA. | |
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MedLine Citation:
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PMID: 21186367 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During immunoglobulin class-switch recombination (CSR), the cytidine deaminase AID induces double-strand breaks into transcribed, repetitive DNA elements called switch sequences. The mechanism that promotes the binding of AID specifically to switch regions remains to be elucidated. Here we used a proteomic screen with in vivo biotinylation of AID to identify the splicing regulator PTBP2 as a protein that interacts with AID. Knockdown of PTBP2 mediated by short hairpin RNA in B cells led to a decrease in binding of AID to transcribed switch regions, which resulted in considerable impairment of CSR. PTBP2 is thus an effector of CSR that promotes the binding of AID to switch-region DNA. |
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Authors:
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Urszula Nowak; Allysia J Matthews; Simin Zheng; Jayanta Chaudhuri |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-12-26 |
Journal Detail:
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Title: Nature immunology Volume: 12 ISSN: 1529-2916 ISO Abbreviation: Nat. Immunol. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-19 Completed Date: 2011-06-14 Revised Date: 2012-05-02 |
Medline Journal Info:
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Nlm Unique ID: 100941354 Medline TA: Nat Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 160-6 Citation Subset: IM |
Affiliation:
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Graduate Program in Immunology and Microbial Pathogenesis, Weill-Cornell Medical School, New York, New York, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Tumor Cell Proliferation Cytidine Deaminase / genetics, immunology, metabolism* DNA / genetics, metabolism* Immunoglobulin Class Switching / genetics Immunoglobulin Switch Region / genetics Mice Mice, Inbred BALB C Mice, Knockout Nerve Tissue Proteins / genetics, immunology, metabolism* Polypyrimidine Tract-Binding Protein / genetics, immunology, metabolism* Protein Binding / genetics RNA, Small Interfering / genetics Transcriptional Activation / genetics, immunology Transgenes / genetics |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI072194/AI/NIAID NIH HHS; R01 AI072194-03/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nerve Tissue Proteins; 0/Ptbp2 protein, mouse; 0/RNA, Small Interfering; 139076-35-0/Polypyrimidine Tract-Binding Protein; 9007-49-2/DNA; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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