Document Detail


A spindle cell variant of diffuse large B-cell lymphoma is characterized by T-cell/ myofibrohistio -rich stromal alterations: analysis of 10 cases and a review of the literature.
MedLine Citation:
PMID:  22738254     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Spindle-shaped diffuse large B-cell lymphoma (Sp-DLBCL) has been recognized as a rare morphologic variant of DLBCL. However, the biological processes that contribute to the specific features of Sp-DLBCL remain poorly understood. In this study, a combined immunophenotypic and genetic analysis was performed in 10 Sp-DLBCL. First, we investigated several unique markers for anaplasia (CD30, ALK, CD68, and EBER-ISH), mesenchyma (SMA, desmin, and vimentin), and B-cell differentiation (CD10, BCL6, and MUM1). We also performed conventional cytogenetic and fluorescence in situ hybridization studies to look for common chromosomal breakpoints (BCL2, BCL6, and MYC). We found that most Sp-DLBCLs were germinal center B cell-like and that none had any other specific phenotypes or any karyotypic abnormalities. Instead, T-cells, CD68-positive macrophages and SMA-positive myofibroblasts were significantly increased in Sp-DLBCL when compared with conventional GCB origin DLBCL cases (n=10) (P=0.012, P<0.001, and P<0.0001 respectively). To further characterize Sp-DLBCL, we next compared the expression of fibroblast growth factor 2 (FGF2) and transforming growth factor-β1 (TGFβ1) between the two types of DLBCL. Finally, we confirmed that the number of FGF2 and TGFβ1-positive stromal cells was markedly increased in Sp-DLBCL and that the difference between these and conventional GCB origin DLBCLs was significant (P<0.0001 and P=0.0017 respectively). Thus, T-cell/myofibrohistio-rich stromal alterations in Sp-DLBCL, especially those mediated by TGFβ1 and FGF2, may play a role in the transition of lymphoma cells into those with spindle-shaped features. © 2012 John Wiley & Sons A/S.
Authors:
Yoshizo Kimura; Fumiko Arakawa; Junichi Kiyasu; Hiroaki Miyoshi; Maki Yoshida; Ayako Ichikawa; Shinji Nakashima; Yukinao Ishibashi; Daisuke Niino; Yasuo Sugita; Ken Ishiyama; Masakazu Higuchi; Yumi Takasaki; Taizo Shimomura; Chisato Koike; Nobuo Kuwahara; Kazuhisa Fujikawa; Koichi Ohshima
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-28
Journal Detail:
Title:  European journal of haematology     Volume:  -     ISSN:  1600-0609     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703985     Medline TA:  Eur J Haematol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Affiliation:
Department of Pathology, School of Medicine, Kurume University, Asahimachi 67, Kurume, Japan.
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