Document Detail

The spinach chloroplast chromosome is bound to the thylakoid membrane in the region of the inverted repeat.
MedLine Citation:
PMID:  1575763     Owner:  NLM     Status:  MEDLINE    
By using a novel fractionation procedure we show that the chloroplast chromosome is specifically bound to the spinach thylakoid in the region of the inverted repeat, near the 16S and 23S rRNA genes. Central to the method is the use of restriction endonucleases, followed by ethylenediaminetetraacetic acid (EDTA) treatment to loosen or uncoil the nucleoid by the removal of specific proteins. The data we present provide a basis for understanding the molecular mechanism of membrane-bound cpDNA function.
J W Liu; R J Rose
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  184     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-06-02     Completed Date:  1992-06-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  993-1000     Citation Subset:  IM    
Department of Biological Sciences, University of Newcastle, New South Wales, Australia.
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MeSH Terms
Chloroplasts / physiology*
Chromosomes / physiology*
DNA / genetics*,  isolation & purification
Edetic Acid / pharmacology
Intracellular Membranes / physiology
Nucleoproteins / isolation & purification
Plant Physiological Phenomena
Plants / genetics*
RNA, Ribosomal, 16S / genetics*
RNA, Ribosomal, 23S / genetics*
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Reg. No./Substance:
0/Nucleoproteins; 0/RNA, Ribosomal, 16S; 0/RNA, Ribosomal, 23S; 60-00-4/Edetic Acid; 9007-49-2/DNA

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