Document Detail


Is spermatozoan acrosin a predictor of fertilization and embryo quality in the human?
MedLine Citation:
PMID:  8224275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate whether acrosin is a more reliable criterion than conventional parameters for assessing semen samples. DESIGN: Total acrosin was estimated biochemically in semen samples obtained for routine screening for infertility and for IVF-ET procedures. SETTING: Assisted Conception Unit, St. James's University Hospital, Leeds, United Kingdom. PATIENTS: Four hundred sixty-three couples being investigated for causes of infertility, and 132 couples undergoing IVF-ET for any indication except antisperm antibodies between 1990 and 1991 were included in the study. INTERVENTIONS: Semen samples were collected as part of routine investigation. Samples from men with consistently high viscosity were collected in alpha-chymotrypsin. MAIN OUTCOME MEASURES: Total spermatozoan acrosin in motile spermatozoa and motile spermatozoan density in couples being assessed for IVF-ET and fertilization and embryo quality in those receiving treatment are considered. RESULTS: Total acrosin levels were less variable (within subject) than either total or motile spermatozoan concentration at ejaculation. Although severely oligozoospermic ejaculates had the lowest levels of total acrosin, overall, there was no significant correlation of spermatozoan concentration between total acrosin levels and percentage fertilization. CONCLUSIONS: Total spermatozoan acrosin activity correlates positively with fertilization rates but not with spermatozoan count. Motile spermatozoan density for insemination may be adjusted to achieve > 7.5 microIU acrosin per oocyte, without compromising fertilization or further embryo development to blastocysts in vitro.
Authors:
R Sharma; J Hogg; D R Bromham
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Fertility and sterility     Volume:  60     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-12-01     Completed Date:  1993-12-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  881-7     Citation Subset:  IM    
Affiliation:
Academic Unit of Obstetrics and Gynaecology, St. James's University Hospital, Leeds, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Acrosin / analysis*
Adult
Biological Markers / analysis
Cell Separation / methods
Centrifugation, Density Gradient / methods
Ejaculation
Embryo Transfer*
Embryo, Mammalian / physiology*
Embryonic and Fetal Development
Female
Fertilization*
Fertilization in Vitro*
Humans
Infertility
Male
Oocytes / physiology*
Sperm Motility*
Spermatozoa / cytology,  enzymology,  physiology*
Chemical
Reg. No./Substance:
0/Biological Markers; EC 3.4.21.10/Acrosin
Comments/Corrections
Comment In:
Fertil Steril. 1994 Sep;62(3):657   [PMID:  8093197 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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