| A spectrophotometric assay for lipase activity utilizing immobilized triacylglycerols. | |
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MedLine Citation:
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PMID: 1779096 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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New substrates for the determination of lipase activity have been developed. Triacylglycerols were immobilized by adsorption on an appropriate carrier or adsorbent yielding a lipase substrate in a powder form. The adsorbed triacylglycerols were easily hydrolyzed by lipases present in a reaction mixture. The released fatty acids were extracted with benzene and converted to the corresponding Cu (II) salts (copper soaps) which were measured spectrophotometrically. |
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Authors:
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I Safarík |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemical and biophysical methods Volume: 23 ISSN: 0165-022X ISO Abbreviation: J. Biochem. Biophys. Methods Publication Date: 1991 Oct-Nov |
Date Detail:
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Created Date: 1992-03-10 Completed Date: 1992-03-10 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 7907378 Medline TA: J Biochem Biophys Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 249-53 Citation Subset: IM |
Affiliation:
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Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Branisovská, Ceské Budĕjovice, Czechoslovakia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Candida / enzymology Lipase / metabolism* Spectrophotometry* Triglycerides / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Triglycerides; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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