| The specificity of pyrimidine nucleoside transport and metabolism by rat jejunum in vitro. | |
| | |
MedLine Citation:
|
PMID: 2778735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
1. 5-Methyluridine perfused through the lumen of isolated loops of rat jejunum gave rise to serosal thymine as the major species transported across the epithelial layer; no serosal 5-methyluridine was detected. 2. At 0.1 mM-luminal 5-methyluridine there was enhanced transmural transport of thymine (P less than 0.001) when compared with either 0.1 mM-thymine or 0.1 mM-thymidine as the luminal substrate for thymine transport. 3. At low luminal concentrations (0.025 mM) thymine was a significantly better substrate (P less than 0.001) for the transport of the free pyrimidine than either of the two nucleosides (thymidine or 5-methyluridine). 4. Luminal deoxyuridine gave rise to uracil as the major species appearing in the serosal secretion. High luminal concentrations of deoxyuridine (0.5 and 1.0 mM) gave rise to low levels of the nucleoside in the serosal fluid. 5. As a consequence of the specificity of the mucosal phosphorolysis the ribonucleosides are favoured over the deoxyribonucleosides as substrates for transmural pyrimidine transport. |
| | |
Authors:
|
J R Bronk; J G Hastewell |
Related Documents
:
|
2742875 - Differences in uptake of high-density lipoproteins by rat adrenals using in vivo vs. in... 7473185 - Membrane permeability and lipophilicity in the isolated perfused rat liver: 5-ethyl bar... 411675 - Acid-base induced alterations in glutamine metabolism and ureogenesis in perfused muscl... 2597105 - The fuel of the spleen. studies using a new method for perfusing the rat spleen with wh... 7093745 - Behavioral effects of systemic injection of ibotenic acid manifested without neuromorph... 834125 - 2-aminoethylphosphonic acid metabolism in the rat. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of physiology Volume: 408 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 1989 Jan |
Date Detail:
|
Created Date: 1989-10-25 Completed Date: 1989-10-25 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0266262 Medline TA: J Physiol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 405-11 Citation Subset: IM |
Affiliation:
|
Department of Biology, University of York. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Transport, Active Deoxyuridine / metabolism Intestinal Mucosa / metabolism Jejunum / metabolism* Male Pyrimidine Nucleosides / metabolism* Rats Rats, Inbred Strains Thymidine / metabolism Thymine / metabolism Uracil / metabolism Uridine / analogs & derivatives, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Pyrimidine Nucleosides; 1463-10-1/ribothymidine; 50-89-5/Thymidine; 58-96-8/Uridine; 65-71-4/Thymine; 66-22-8/Uracil; 951-78-0/Deoxyuridine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Responses of electroreceptors in the platypus bill to steady and alternating potentials.
Next Document: Systemically administered glycine protects against strychnine convulsions, but not the behavioural e...