Document Detail


APC/CCdh1 specific degradation of Hsl1 and Clb2 is required for proper stress responses of S. cerevisiae.
MedLine Citation:
PMID:  19713762     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cdh1 activates the Anaphase Promoting Complex/Cyclosome (APC/C(Cdh1)) throughout G(1) to degrade key cell cycle proteins. Cdh1 is not essential for cell proliferation, in spite of the fact that overexpression of some its degradation substrates is highly toxic. We report here that cdh1Delta cells are sensitive to stresses that activate the CWI (Cell Wall Integrity) and Hog1 MAP kinase pathways. Stresses did not activate APC/C(Cdh1) and cellular sensitivity was thus clearly due to constitutively elevated substrate levels. To explore the contribution of stabilization of individual APC/C(Cdh1) substrates to stress sensitivity, we generated cell lines expressing stabilized substrate mutants under their endogenous promoters. Cells expressing stabilized Hsl1 were sensitive to caffeine and failed to activate the Slt2 pathway. Cells expressing partially stable Clb2 were particularly sensitive to different stresses, possibly due to reduced Sic1 levels. Cells expressing stabilized Cdc5 were much less stress sensitive. Interestingly sensitivity of cdh1Delta cells does not seem to be restricted to G(1) but is manifested also during S and G(2) when the APC/C(Cdh1) is inactive anyway. We thus hypothesize that a role of G(1) specific APC/C(Cdh1) activity is to reset substrate levels to enables appropriate regulation of substrate accumulation in the subsequent phases of the cell cycle.
Authors:
Kobi J Simpson-Lavy; Julia Sajman; Drora Zenvirth; Michael Brandeis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-25
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  8     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-09     Completed Date:  2009-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3003-9     Citation Subset:  IM    
Affiliation:
The Department of Genetics, The Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
Cell Cycle
Cyclin B / metabolism*
G1 Phase
Mitogen-Activated Protein Kinases / metabolism
Protein Stability
Protein-Serine-Threonine Kinases / metabolism*
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae Proteins / metabolism*,  physiology*
Stress, Physiological
Ubiquitin-Protein Ligase Complexes / physiology*
Chemical
Reg. No./Substance:
0/CLB2 protein, S cerevisiae; 0/Cyclin B; 0/Hct1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC 2.7.11.1/HSL1 protein, S cerevisiae; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.24/SLT2 protein, S cerevisiae; EC 6.3.2.19/Ubiquitin-Protein Ligase Complexes; EC 6.3.2.19/anaphase-promoting complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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