Document Detail


A specific arginase-based assay for L-canavanine in leguminous plants.
MedLine Citation:
PMID:  7153457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A convenient method for the analysis of free L-canavanine in leguminous plants is described. Canavanine was specifically hydrolyzed to canaline and urea by the enzyme arginase (EC 3.5.3.1). The resulting amino-oxy functions of canaline were measured based on their ability to bleach the yellow colour of pyridoxal 5'-phosphate. Canavanine in the seeds of Canavalia ensiformis was determined with this method.
Authors:
T K Korpela; H Lorenz; S Laakso
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  7     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2001-11-28    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  67-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arginase / metabolism*
Canavanine / analysis*
Fabaceae / analysis*
Plants, Medicinal*
Spectrophotometry / methods
Chemical
Reg. No./Substance:
543-38-4/Canavanine; EC 3.5.3.1/Arginase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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