Document Detail

The spatial variation of the refractive index in biological cells.
MedLine Citation:
PMID:  8778819     Owner:  NLM     Status:  MEDLINE    
With a phase microscope the phase shift of cells from type L 929 fibroblast and mitochondria from liver cells was measured. Compared to the total phase shift caused by the cell relative to vacuum (approximately 1400 nm) the single phase shift of the mitochondria (approximately 180 nm) is small. Only the nucleus and the membrane of the cell give a visibly different phase shift relative to the mean value of the cell. The Fraunhofer diffraction of the measured phase object is calculated. With a simplified scattering theory, i.e. Rayleigh-Gans Scattering, different phase objects are investigated and their differential cross section is discussed.
J Beuthan; O Minet; J Helfmann; M Herrig; G Müller
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Physics in medicine and biology     Volume:  41     ISSN:  0031-9155     ISO Abbreviation:  Phys Med Biol     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-09-19     Completed Date:  1996-09-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0401220     Medline TA:  Phys Med Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  369-82     Citation Subset:  IM    
Institute for Medical-Technical Physics and Laser Medicine, Freie Universität Berlin, Germany.
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MeSH Terms
Biophysical Phenomena
Cell Line
Liver / ultrastructure
Microscopy, Phase-Contrast
Mitochondria / ultrastructure
Models, Biological
Optics and Photonics*
Scattering, Radiation

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