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The spatial organization and extraction of the wall-forming bodies of Eimeria maxima.
MedLine Citation:
PMID:  23622252     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
SUMMARY Eimeria maxima has been used as a model apicomplexan parasite to study sexual stage development and oocyst wall formation. A complete understanding of the wall's biochemical and biophysical properties is of great interest in research on all apicomplexan parasites. Purified gametocytes, zygotes and oocysts were analysed by three-dimensional confocal microscopy, and wide-field fluorescent microscopy was used to investigate the appearance and spatial organization of the 2 types of wall-forming bodies (WFBs). In addition, a variety of staining procedures and immunoassays were used to assess the biosynthesis, metabolic activity, intactness and molecular composition of the WFBs in situ. WFBs were extracted from gametocytes/zygotes and their composition was assessed by microscopy and SDS-PAGE analysis. It was concluded that isolated gametocytes are intact and metabolically active. Additionally, it was observed that the Type 1 WFBs are aligned at the periphery of the parasite and fuse together producing neutral lipid rich patches that appear to be inserted into the space between 2 parasite-specific membranes. Finally, it was shown that the WFBs extracted from purified gametocytes had the same shape, size and staining properties as those observed in situ, and contained the major glycoprotein antigens known to be present in these organelles.
Authors:
Sonja Frölich; Michael Johnson; Michelle Robinson; Rolf Entzeroth; Michael Wallach
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Parasitology     Volume:  140     ISSN:  1469-8161     ISO Abbreviation:  Parasitology     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401121     Medline TA:  Parasitology     Country:  England    
Other Details:
Languages:  eng     Pagination:  876-87     Citation Subset:  IM    
Affiliation:
The iThree Institute, School of Medical and Molecular Biosciences, University of Technology Sydney, PO Box 123, Broadway, Sydney, New South Wales, 2007, Australia.
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