Document Detail

The sonodynamic antitumor effect of methylene blue on sarcoma180 cells in vitro.
MedLine Citation:
PMID:  19528509     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Sonodynamic therapy (SDT) is a promising methodology for cancer treatment. Methylene blue (MB) is a phenothiazine dye that is widely used in clinical practice and can be administered intravenously. MATERIALS AND METHODS: The sonodynamic antitumor effect of 1, 10 and 100 microM MB on sarcoma180 (S180) cells was investigated in vitro. RESULTS: After ultrasound (US) exposure at 0.24 W/cm(2) for 30 seconds, survival rates of S180 cells in the presence of 10 and 100 microM MB were significantly lower than that of the control group. These effects were significantly inhibited by the addition of D-mannitol, but not by L-histidine or superoxide dismutase. Microvilli loss and blebbing on the surface of S180 cells were observed in the presence of 100 muM MB after US exposure. CONCLUSION: MB has a sonodynamic antitumor effect on S180 cells in vitro and the hydroxyl radical appears to be the principal mediator of this effect.
Chiyo Komori; Kyoji Okada; Koichi Kawamura; Shuichi Chida; Toshio Suzuki
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  29     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-16     Completed Date:  2009-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2411-5     Citation Subset:  IM    
Department of Pharmaceutical Science, Akita University Hospital, Akita 010-8543, Japan.
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MeSH Terms
Cell Survival / drug effects
Enzyme Inhibitors / pharmacology*
Mannitol / pharmacology
Methylene Blue / pharmacology*
Mice, Inbred ICR
Oxygen / metabolism
Sarcoma 180 / therapy*
Sweetening Agents / pharmacology
Tumor Cells, Cultured
Ultrasonic Therapy*
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Sweetening Agents; 61-73-4/Methylene Blue; 69-65-8/Mannitol; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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