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A solution (17)O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase.
MedLine Citation:
PMID:  23323266     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Solution (17)O-NMR application to biological macromolecules is extremely limited. We describe here (17)O-NMR observation of the (17)O(2)-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective (17)O(2) oxidation. (17)O-NMR with the aid of (17)O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.
Authors:
Shinya Hanashima; Noriko Fujiwara; Kana Matsumoto; Noriyuki Iwasaki; Guo-Qing Zheng; Hidetaka Torigoe; Keiichiro Suzuki; Naoyuki Taniguchi; Yoshiki Yamaguchi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Chemical communications (Cambridge, England)     Volume:  -     ISSN:  1364-548X     ISO Abbreviation:  Chem. Commun. (Camb.)     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610838     Medline TA:  Chem Commun (Camb)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Structural Glycobiology Team, RIKEN ASI, 2-1 Hirosawa, Wako-shi, 351-0198 Saitama, Japan. yyoshiki@riken.jp.
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