Document Detail

A soluble molecule secreted by human blastocysts modulates regulation of HOXA10 expression in an epithelial endometrial cell line.
MedLine Citation:
PMID:  14607569     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine the existence of a soluble signal, secreted from the human blastocyst embryo, that induces HOXA10 gene expression before cell-cell contact. DESIGN: To analyze, by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), cell-free media that had contained human embryos cultured to the blastocyst stage for a soluble molecule that induces HOXA10 expression in an endometrial epithelial cell line (Ishikawa). SETTING: Assisted reproduction technology program of Yale University, New Haven, Connecticut. PATIENT(S): Patients undergoing intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) cycles. Treatment of Ishikawa cells with blastocyst-conditioned media. MAIN OUTCOME MEASURE(S): Determination of HOXA10 gene expression. RESULT(S): We demonstrate that cell-free media that had contained human embryos cultured to the blastocyst stage contain a soluble molecule that induces HOXA10 expression in an endometrial epithelial cell line (Ishikawa). We found that hCG does not induce HOXA10 in Ishikawa cells. CONCLUSION(S): Soluble molecules induce a well-characterized marker of endometrial receptivity in endometrial cells without blastocyst apposition. Additionally, HOXA10 induction can serve as a means of evaluating human embryos cultured for IVF and ET. High quality embryos may induce local endometrial receptivity before trophectoderm-endometrial contact.
Denny Sakkas; Christina Lu; Ebru Zulfikaroglu; Evelyn Neuber; Hugh S Taylor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Fertility and sterility     Volume:  80     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-10     Completed Date:  2003-12-19     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1169-74     Citation Subset:  IM    
Yale University School of Medicine, Department of Obstetrics and Gynecology, New Haven, Connecticut 06520, USA.
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MeSH Terms
Biological Factors / chemistry,  physiology*
Blastocyst / metabolism*
Cell Line
Chorionic Gonadotropin / pharmacology,  physiology
Culture Media, Conditioned / pharmacology
Embryonic and Fetal Development / physiology
Endometrium / cytology,  drug effects,  metabolism*
Epithelial Cells / drug effects,  metabolism
Fertilization in Vitro
Homeodomain Proteins / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Sperm Injections, Intracytoplasmic
Reg. No./Substance:
0/Biological Factors; 0/Chorionic Gonadotropin; 0/Culture Media, Conditioned; 0/Homeodomain Proteins; 164384-16-1/Hoxa10 protein, mouse

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