Document Detail


A solid-phase luminescent immunoassay for human chorionic gonadotropin using Pholas dactylus bioluminescence.
MedLine Citation:
PMID:  3701068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A solid-phase luminescent immunoassay (LIA), based on the light emission produced as a result of the oxidation of Pholas dactylus luciferin by horseradish peroxidase (HRP) in the presence of molecular oxygen, was developed for human chorionic gonadotropin (hCG). The light emitted in the presence of diethyldithiocarbamate permitted the detection of 0.1 fmol HRP. HRP retained most of its light-emitting capacity after coupling with purified anti-hCG antibody by glutaraldehyde. The LIA involved immobilization of the antigen in plastic tubes coated with purified anti-hCG antibody and detection of the immunocomplex by light emission in the presence of Pholas luciferin. Light emission was linear for antigen concentration within the range 0.5-100 ng/ml. LIA correlates reasonably well with RIA and has a comparable sensitivity (0.5 ng hCG/ml).
Authors:
A Longeon; J P Henry; R Henry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunological methods     Volume:  89     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-06-10     Completed Date:  1986-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  103-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bivalvia
Blood
Chorionic Gonadotropin / analysis*
Firefly Luciferin / diagnostic use*
Horseradish Peroxidase / metabolism
Humans
Immunoassay / methods*
Isoenzymes / metabolism
Luminescent Measurements
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Isoenzymes; 2591-17-5/Firefly Luciferin; EC 1.11.1.-/Horseradish Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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