Document Detail


A solid-phase fluoroimmunoassay for the quantitation of antibodies to Toxoplasma gondii.
MedLine Citation:
PMID:  6994935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A solid-phase fluoroimmunoassay (FIAX¿) for the detection and quantitation of human antibodies to Toxoplasma gondii is described. The method is a modification of the procedure of Walls and Barnhart [1] which differs from the earlier test in that it uses a dual rather than single surface StiQTM Sampler, having both an antigen and a control surface. Each sampler is carried through four steps: reaction with diluted serum, a buffer wash, reaction with FITC-labeled goat anti-human IgG, and a final buffer wash. The fluorescence of each surface is measured using a dedicated surface-reading fluorometer. Serum titers are interpolated from a standard curve. The antibody titers of 233 sera were determined by both the indirect immunofluorescent antibody (IFA) and the FIAX¿ assays. The titers determined by the two tests agreed within one four-fold dilution for 226 (97%) of the sera. For replicates (n = 21) of the sera with low, mid and high antibody titers the coefficients of variation were 13.6, 10.0, and 12.3% respectively.
Authors:
B Hyde; M W Burgett; E T Maggio
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  103     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1980 May 
Date Detail:
Created Date:  1980-10-27     Completed Date:  1980-10-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  393-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies / analysis*
Fluorescent Antibody Technique*
Humans
Toxoplasma / immunology*
Toxoplasmosis / immunology*
Chemical
Reg. No./Substance:
0/Antibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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