Document Detail


The snoRNA box C/D motif directs nucleolar targeting and also couples snoRNA synthesis and localization.
MedLine Citation:
PMID:  9649444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Most small nucleolar RNAs (snoRNAs) fall into two families, known as the box C/D and box H/ACA snoRNAs. The various box elements are essential for snoRNA production and for snoRNA-directed modification of rRNA nucleotides. In the case of the box C/D snoRNAs, boxes C and D and an adjoining stem form a vital structure, known as the box C/D motif. Here, we examined expression of natural and artificial box C/D snoRNAs in yeast and mammalian cells, to assess the role of the box C/D motif in snoRNA localization. The results demonstrate that the motif is necessary and sufficient for nucleolar targeting, both in yeast and mammals. Moreover, in mammalian cells, RNA is targeted to coiled bodies as well. Thus, the box C/D motif is the first intranuclear RNA trafficking signal identified for an RNA family. Remarkably, it also couples snoRNA localization with synthesis and, most likely, function. The distribution of snoRNA precursors in mammalian cells suggests that this coupling is provided by a specific protein(s) which binds the box C/D motif during or rapidly after snoRNA transcription. The conserved nature of the box C/D motif indicates that its role in coupling production and localization of snoRNAs is of ancient evolutionary origin.
Authors:
D A Samarsky; M J Fournier; R H Singer; E Bertrand
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The EMBO journal     Volume:  17     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-08-21     Completed Date:  1998-08-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3747-57     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Binding Sites
COS Cells
Cell Nucleus / metabolism
Humans
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Fungal / biosynthesis*,  chemistry,  metabolism
RNA, Small Nuclear / biosynthesis*,  chemistry,  metabolism
Saccharomyces cerevisiae / genetics*
Grant Support
ID/Acronym/Agency:
GM 57071/GM/NIGMS NIH HHS; GM19351/GM/NIGMS NIH HHS; GM54887/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Fungal; 0/RNA, Small Nuclear
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