| sn-position determination of phospholipid-linked fatty acids derived from erythrocytes by liquid chromatography electrospray ionization ion-trap mass spectrometry. | |
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MedLine Citation:
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PMID: 15884770 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The sn-position of FA in membrane lipids has an influence on the physiological function of cells, is predictive for diseases, and therefore is useful for diagnostics. The current study compares the compositions of acyl chain substituents in the sn-1 and sn-2 positions of the glycerol backbones of phospholipids derived from human erythrocytes by using RP-HPLC coupled with on-line electrospray ionization ion trap MS. Preferential loss of the acyl group in the sn-1 position was used to determine the degree of regiospecific preference exhibited by the phospholipid molecules. The identities of the molecular species and the positions of the acyl substituents were identified using product-ion spectra of major precursor ions selected from the mass spectra averaged across peaks in the total ion chromatogram. Saturated FA were found to be located mainly in the sn-1 position of the glycerol backbones of erythrocyte phospholipids, whereas PUFA were found primarily in the sn-2 position. All measured phospholipids revealed palmitic acid (16:0) at the sn-1 position. Linoleic acid (18:2n-6) and arachidonic acid (20:4n-6) were found to be attached exclusively to the sn-2 position of the backbone, whereas eicosadienoic (20:2n-6) and eicosatrienoic acid (20:3n-9) occurred in both positions of the backbone of PC. Oleic (18:1n-9), linoleic (18:2n-6), and octadecatrienoic (18:3) acids of PE and PS were linked to both positions. Lignoceric acid (24:1 n-9) was found to be strictly localized at the sn-2 position, whereas nervonic (24:1n-9) acid of PS was associated with both positions of the backbone. A detailed analysis of the blood cell membrane lipids by MS might be helpful to characterize postprandial kinetics of pharmacological or dietary lipid applications, as well as environmental influences on cell membranes. |
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Authors:
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Christopher Beermann; Michael Möbius; Nadine Winterling; Joachim J Schmitt; Günther Boehm |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 40 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-05-11 Completed Date: 2005-09-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 211-8 Citation Subset: IM |
Affiliation:
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Numico Research Germany, D-61381 Friedrichsdorf, Germany. milupa.research@T-online.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid
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methods Erythrocytes / chemistry* Fatty Acids / chemistry*, metabolism Humans Phospholipids / chemistry*, metabolism Spectrometry, Mass, Electrospray Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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