Document Detail


sn-1,2-Diacylglycerols and phorbol diesters stimulate thromboxane synthesis by de novo synthesis of prostaglandin H synthase in human promyelocytic leukemia cells.
MedLine Citation:
PMID:  3102559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the regulation of thromboxane (TX) synthesis in promyelocytic leukemia cells during macrophage differentiation. Cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) showed rates of TXB2 synthesis from exogenous arachidonic acid that exceeded that of control cells by a factor of up to 81. Cells treated with sn-1,2-dioctanoylglycerol (diC8) showed similarly high TXB2 synthesis rates when diC8 was added concomitantly with a subthreshold concentration of TPA or when given in multiple doses. These activities depended on de novo synthesis of prostaglandin H (PGH) synthase because: microsomal PGH synthase activity showed large increases in Vmax values, and mass measurements of PGH synthase revealed the presence of PGH synthase in differentiating cells whereas the enzyme was undetectable in control cells. These results indicate that macrophage differentiation is associated with stimulation of TXB2 synthesis that requires both activation of protein kinase C and de novo synthesis of PGH synthase.
Authors:
M Goerig; A J Habenicht; R Heitz; W Zeh; H Katus; B Kommerell; R Ziegler; J A Glomset
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  79     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1987-04-06     Completed Date:  1987-04-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  903-11     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Arachidonic Acid
Arachidonic Acids / metabolism
Calcimycin / pharmacology
Cell Differentiation
Cell Line
Diglycerides / pharmacology*
Glycerides / pharmacology*
Humans
Kinetics
Leukemia, Myeloid / metabolism*
Macrophages / metabolism
Prostaglandin-Endoperoxide Synthases / biosynthesis*
Protein Kinase C / metabolism
Tetradecanoylphorbol Acetate / pharmacology*
Thromboxane B2 / biosynthesis*
Trifluoperazine / pharmacology
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Diglycerides; 0/Glycerides; 1069-87-0/1,2-dioctanoylglycerol; 117-89-5/Trifluoperazine; 16561-29-8/Tetradecanoylphorbol Acetate; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 54397-85-2/Thromboxane B2; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 2.7.11.13/Protein Kinase C
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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