| The smallest active fragment of microtubule-associated protein 4 and its interaction with microtubules in phosphate buffer. | |
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MedLine Citation:
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PMID: 22451409 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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To analyze the interaction between microtubule-associated protein (MAP) 4 and microtubules physicochemically, a MAP4 active site fragment was designed for nuclear magnetic resonance (NMR) use. The fragment was bacterially expressed and purified to homogeneity. The buffer conditions for NMR were optimized to support microtubule assembly. The fragment was found to bind to microtubules under the optimized buffer conditions. |
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Authors:
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Yurika Hashi; Lisa Nagase; Kazuyuki Matsushima; Susumu Kotani |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 76 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-03-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 601-4 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Faculty of Science, Kanagawa University. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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